Liver (Human) Tissue Lysate - adult normal tissue (ab29889)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notes Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab29889 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    References for Liver (Human) Tissue Lysate - adult normal tissue (ab29889)

    This product has been referenced in:
    • Jaacks LM  et al. Placental expression of the heme transporter, feline leukemia virus subgroup C receptor, is related to maternal iron status in pregnant adolescents. J Nutr 141:1267-72 (2011). Read more (PubMed: 21593354) »

    See 1 Publication for this product

    Product Wall

    Thank you very much for your call yesterday and for your patience while I've looked into your enquiry.

    We have 5 liver lysates that are from different donors:

    Ab44563-

    http://www.abcam.com/Liver-Human-Tissue-Lysate-adult-n...

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    Thank you for calling Abcam today.

    I am sorry about the issues that you have been having with ab13868 in western blot. AS we discussed today, I am sending you a vial of human liver lysate to use as a positive control (although I am sending ab2...

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    Thank you for your enquiry

    We obtain this product from an external source and unfortunately they are not able to provide specific information about the sample preparation.

    I reviewed the literature and found the following protocol...

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    Thank you for your enquiry and your valuable feedback. We will change the shipping conditions for these products as soon as possible. This is to confirm that we will ship again one vial of each of these lysates as free of charge replacements - on d...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"