Liver (Human) Tissue Lysate - adult normal tissue (ab44477)

Overview

• Product nameLiver (Human) Tissue Lysate - adult normal tissueSee all Liver lysates ...
• General notesDiagnosis: Normal Sex: Male Age: 32 years Insoluble protein fraction

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferConstituents: 0.1% SDS, PBS, 5M Urea, 2M Thiourea, 50mM DTT, pH 7.4
  Please note that 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate, as a result the lysate may not be suitable for applications which require native protein in a non-denaturing buffer. The final Laemmli buffer concs are: 31.25mM Tris HCl, pH 6.8, 12.5% Glycerol, 1% SDS, 0.005% Bromophenol Blue, 25mM TCEP.
• Concentration information loading...
• Purification NotesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
• Lysate notes This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44477: Extraction 1: soluble protein fraction ab44476 Human Liver Tumor Tissue lysate ab44475 Human Liver Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44478 Human Liver Tumor Tissue lysate ab44477 Human Liver Normal Tissue lysate (matched)
• Research Areas