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- Proteins and Peptides
- Secondary antibodies
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Product name
Anti-Livin antibody
See all Livin products (9) ...
Description
Rabbit polyclonal to Livin
Specificity
Detects both the alpha and beta (short and long) isoforms of Livin.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: CRAPVRSRVRTFLS, corresponding to amino acids 264 - 280 (short form) or 281 - 298 (long form) of Human Livin.
CRAPVRSRVRTFLS
Positive control
WB: Raji cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> IAPs
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
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Western blot - Livin antibody (ab5393)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 2 - 4 µg/ml. Detects bands of approximately 30 kDa (possibly short form) + 33 kDa (long form).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis.
Tissue specificity
Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes.
Sequence similarities
Belongs to the IAP family.
Contains 1 BIR repeat.
Contains 1 RING-type zinc finger.
Cellular localization
Nucleus. Cytoplasm. Nuclear, and in a filamentous pattern throughout the cytoplasm.
Target information above from: UniProt accessionQ96CA5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Baculoviral IAP repeat containing 7 antibody
- Baculoviral IAP repeat containing protein 7 antibody
- Baculoviral IAP repeat-containing protein 7 antibody
see all
Database links
- Entrez Gene: 79444 Human
- Omim: 605737 Human
- SwissProt: Q96CA5 Human
- Unigene: 256126 Human
Anti-Livin antibody images:
Western blot - Livin antibody (ab5393)
Anti-Livin antibody (ab5393) at 4 µg/ml + Human Raji cell lysate
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
References for Anti-Livin antibody (ab5393)
ab5393 has not yet been referenced specifically in any publications.
Publishing research using ab5393? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"