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Product name
Luminescent ATP Detection Assay Kit
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Product overview
Total levels of cellular ATP can be used to assess cell viability, cell proliferation and cytotoxicity of a wide range of compounds and biological response modifiers. ab113849 irreversibly inactivates ATP degrading enzymes (ATPases) during the lysis step, ensuring that the luminescent signal obtained truly corresponds to the endogenous levels of ATP. The major advantages to this luminescence assay are:
Long luminescence signal: The half life of the luminescent signal is greater than 5 hours and therefore a special luminometer with injectors is not required.
Fast: Results are obtained in less than 30 minutes.
Simple: There are no separation steps and there are only 2 reagent additions.
Homogenous: No cell harvesting or centrifugation required.
Sensitive: The limit of detection is 5 cells in 100µL.
Wide linear dynamic range: Approximately from 1pM to 1µM of ATP (this will vary depending on instrument sensitivity).
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at +4°C.
| Components | Identifier | 300 tests |
|---|---|---|
| Detergent | P/N 8206000 | 1 x 20ml |
| Lyophilized ATP standard | P/N 8206003 | 1 vial |
| Lyophilized substrate | P/N 8206002 | 3 vials |
| Substrate Buffer | P/N 8206001 | 1 x 20ml |
Research areas
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Cell Viability and Senescence Kits
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Kits/ Lysates/ Other >> Kits >> Cell Damage Kits
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Functional Studies - Luminescent ATP Detection Assay Kit (ab113849)
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Relevance
ATP (Adenosine-5'-triphosphate) is a multifunctional nucleotide that is most important in intracellular energy transfer. Cell death (especially apoptosis) is an energy-dependent process that requires ATP. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions, the cell will die. A typical apoptotic cell exhibits a significant decrease in ATP level. Therefore, loss of ATP level in cells has been used as an indicator of cell death. In contrast, cell proliferation has been recognized by increased levels of ATP.
Alternative names
- Adenosine 5' triphosphate
- ms957
Applications
Show applications key
Our Abpromise guarantee covers the use of ab113849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS
Luminescent ATP Detection Assay Kit images:
Functional Studies - Luminescent ATP Detection Assay Kit (ab113849)
ab113849 ATP detection kit cytotoxicity data. 25000 HepG2 cells were seeded into each well, allowed to adhere and treated for 4 hours with 25µM rotenone and vehicle control (DMSO) in glucose based complete media. After treatment, cells were lysed, exposed to the ATP substrate solution and signal was measured on a luminescent counter. Mean and standard deviation is plotted for 3 replicates from each condition. Rotenone induces cytotoxicity in HepG2 cells.
Protocols
References for Luminescent ATP Detection Assay Kit (ab113849)
ab113849 has not yet been referenced specifically in any publications.
Publishing research using ab113849? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"