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Product Name
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Lung (Mouse) Whole Cell Lysate - normal tissue, 0 days old
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Product type
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Lysates
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Description
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Lung (Mouse) Whole Cell Lysate - normal tissue, 0 days old
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Tested applications
(see key)
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WB
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Application notes
(see key)
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Recommended dilutions
Mouse lung tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.
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Research areas
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Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Mouse >> Respiratory
Kits/ Lysates/ Other >> Lysates >> Tissue Lysates >> Mouse >> Respiratory
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Relevance
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The mouse lung tissue was collected from zero (0) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
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Storage buffer
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Preservative: None
Constituents: 5% Beta mercaptoethanol, SDS-PAGE sample loading buffer 1X Material safety datasheets (MSDS) for this product:
Mercaptoethanol MSDS
Sodium dodecyl sulfate MSDS
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Source: Outbred NIH-Swiss Albino mice (0 days old)
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