Recombinant Anti-Luteinizing Hormone beta antibody [EPR8195] (ab174265)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8195] to Luteinizing Hormone beta
- Suitable for: mIHC, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Luteinizing Hormone beta antibody [EPR8195]
See all Luteinizing Hormone beta primary antibodies -
Description
Rabbit monoclonal [EPR8195] to Luteinizing Hormone beta -
Host species
Rabbit -
Tested applications
Suitable for: mIHC, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human pancreas tissue. mIHC: Human pituitary gland.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8195 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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mIHC |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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mIHC
Use at an assay dependent concentration. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. -
Tissue specificity
Pituitary gland. -
Involvement in disease
Defects in LHB are a cause of hypogonadism LHB-related (HGON-LHB) [MIM:152780]. Hypogonadism is characterized by infertility and pseudohermaphroditism. -
Sequence similarities
Belongs to the glycoprotein hormones subunit beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3972 Human
- Omim: 152780 Human
- SwissProt: P01229 Human
- Unigene: 154704 Human
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Alternative names
- CGB4 antibody
- CHORIONIC GONADOTROPIN, BETA POLYPEPTIDE 4 antibody
- hLHB antibody
see all
Images
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This data was developed using ab221494, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pituitary gland labelling ACTH with ab233954 at 1/5000 dilution (0.214 μg/ml) (B), Luteinizing Hormone beta with ab221494 at 1/4000 dilution (0.258 μg/ml) (C) and Growth Hormone with ab155276 at 1/5000 dilution (0.044 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-Growth Hormone (gray; Opal™690), anti-ACTH (green; Opal™520) and anti-Luteinizing Hormone beta (red; Opal™570) on human pituitary gland.
Panel B: anti-ACTH stained on corticotrophs.
Panel C: anti-Luteinizing Hormone beta stained on gonadotrophs.
Panel D: anti-Growth Hormone stained on somatotrophs.The section was incubated in three rounds of staining: in the order of ab155276, ab233954, and ab221494 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Luteinizing Hormone beta with ab174265 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab174265 has not yet been referenced specifically in any publications.