Lymphotactin antibody (ab10607)

Product Name 

Lymphotactin antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to Lymphotactin

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu

Specificity 

Anti-Human Lymphotactin recognizes recombinant human lymphotactin by various immunochemical techniques including neutralization, immunoblotting, and ELISA. The antibody does not neutralize the biological activity of recombinant mouse lymphotactin. Based on immunoblotting, this antibody shows less than 2% cross-reactivity with recombinant mouse lymophotactin.

Tested applications 
(see key)

ELISA, Neut, WB

Abreviews 

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant human lymphotactin is approximately 0.3 ng/well.
Neut: Use at a concentration of 20 - 30 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. The detection limit for recombinant human lymphotactin is approximately 5 ng/lane under non-reducing and reducing conditions.Predicted molecular weight: 13.6 kDa.

To measure the ability of anti-human lymphotactin to neutralize the bioactivity of recombinant human lymphotactin for BaF/3 human XCR-1 transfected cells, recombinant human lymphotactin is incubated with various concentrations of the antibody for 15 minutes at room temperature in a 96 well plate. Following this pre-incubation, 75 µl of the cytokine-antibody solution (containing recombinant human lymphotactin at a final concentration of 0.5 µg/ml and antibody at concentrations from 0.1-200 µg/ml) is transferred to the lower compartment of a 96 well chemotaxis chamber. The chemotaxis chamber is then assembled using a PVP-free polycarbonate filter (5 micron size) and 2 x 10⁶ cells/well is added to the top chamber. Following incubation for 3 hours at 37 °C in a 5% CO₂ humidified incubator, the chamber is carefully disassembled. The cells that migrate through to the lower chamber are transferred to a 96 well plate. Chemotaxis is measured by Alamar Blue staining of cells that have migrated through the filter.

The Neutralization Dose50 (ND50) for anti-human lymphotactin is approximately 20 to 30 µg/ml in the presence of 0.5 µg/ml of recombinant human lymphotactin using BaF/3 cells transfected with recombinant human XCR-1. The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit a maximum response. The exact concentration of antibody required to neutralize recombinant human lymphotactin activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Immunology >> Innate Immunity >> Chemokines >> Gamma Chemokines (C)

Relevance 

Human lymphotactin (Lptn)/XCL1, also named human SCM1 and ATAC, is a member of gamma or C subfamily of chemokines that lack two of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended C-terminus. Human lymphotactin encodes a 114 amino acid precursor protein with a 21 amino acid predicted signal peptide. The gene for human and mouse lymphotactin has been mapped to chromosome 1. Human and mouse lymphotactin share approximately 60% amino acid sequence identity.

The expression of lymphotactin is restricted to activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. Since lymphotactin is produced by lymphocytes and acts on lymphocytes, it is speculated that it is a messenger in T cell chemoattraction. Human lymphotactin is chemotactic for lymphocytes and NK cells. Its expression is abundant in some activated T cells such as activated CD8+ T cells and other class I MCH restricted T cells and is absent in CD4+ T cells. It has been shown that lymphotactin is a key regulator of lymphocyte motility and adhesion during acute allograft rejection. The specific receptor for lymphotactin is the orphan receptor XCR1 (GPR5).

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: PBS

Purification notes 

Affinity isolated antigen specific antibody is obtained from goat anti-lymphotactin antiserum by immuno-specific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the peptide.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Endotoxin: < 10 ng/mg antibody as determined by the Limulus amebocyte lysate (LAL) method.

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