Lymphotactin antibody (ab9686)

Product Name 

Lymphotactin antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Lymphotactin

Immunogen 

Highly pure (>98%) recombinant human Lymphotactin

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, Neut, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
Neutralization:To yield one-half maximal inhibition [ND₅₀] of the biological activity of human Lymphotactin (60.00 ng/ml), a concentration of 1.7 - 3.0 µg/ml of this antibody is required.

ELISA:To detect human Lymphotactin by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant human Lymphotactin.

Western Blot:To detect human Lymphotactin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Lymphotactin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Cellular localization 

Secreted

Research areas 

Immunology >> Innate Immunity >> Chemokines >> Gamma Chemokines (C)

Relevance 

Human lymphotactin (Lptn)/XCL1, also named human SCM1 and ATAC, is a member of gamma or C subfamily of chemokines that lack two of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended C-terminus. Human lymphotactin encodes a 114 amino acid precursor protein with a 21 amino acid predicted signal peptide. The gene for human and mouse lymphotactin has been mapped to chromosome 1. Human and mouse lymphotactin share approximately 60% amino acid sequence identity.

The expression of lymphotactin is restricted to activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. Since lymphotactin is produced by lymphocytes and acts on lymphocytes, it is speculated that it is a messenger in T cell chemoattraction. Human lymphotactin is chemotactic for lymphocytes and NK cells. Its expression is abundant in some activated T cells such as activated CD8+ T cells and other class I MCH restricted T cells and is absent in CD4+ T cells. It has been shown that lymphotactin is a key regulator of lymphocyte motility and adhesion during acute allograft rejection. The specific receptor for lymphotactin is the orphan receptor XCR1 (GPR5).

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Purity 

Immunogen affinity purified

Storage buffer 

PBS, pH 7.4, no preservative, sterile filtered

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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