Lymphotactin protein (ab9687)

Product Name 

Lymphotactin protein

Product type 

Proteins and Peptides

Peptide 

Highly pure (>98%) recombinant human Lymphotactin.

Tested applications 
(see key)

FuncS, SDS-PAGE

Application notes 
(see key)

Recommended dilutions
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
This peptide can be used with studies using ab9686.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Immunology >> Innate Immunity >> Chemokines >> Gamma Chemokines (C)

Relevance 

Human lymphotactin (Lptn)/XCL1, also named human SCM1 and ATAC, is a member of gamma or C subfamily of chemokines that lack two of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended C-terminus. Human lymphotactin encodes a 114 amino acid precursor protein with a 21 amino acid predicted signal peptide. The gene for human and mouse lymphotactin has been mapped to chromosome 1. Human and mouse lymphotactin share approximately 60% amino acid sequence identity.

The expression of lymphotactin is restricted to activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. Since lymphotactin is produced by lymphocytes and acts on lymphocytes, it is speculated that it is a messenger in T cell chemoattraction. Human lymphotactin is chemotactic for lymphocytes and NK cells. Its expression is abundant in some activated T cells such as activated CD8+ T cells and other class I MCH restricted T cells and is absent in CD4+ T cells. It has been shown that lymphotactin is a key regulator of lymphocyte motility and adhesion during acute allograft rejection. The specific receptor for lymphotactin is the orphan receptor XCR1 (GPR5).

Storage buffer 

n/a

Purification notes 

Sterile filtered
Greater than 98% pure by SDS-PAGE and HPLC analyses.
Endotoxin level is less than 0.1 ng per µg (1EU/µg). The biological activity of this product was determined by its ability to chemoattract human T cells using a concentration of 10.0-100.0 ng/ml.

Form 

Lyophilised

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

This product was found to mediate chemotaxis of purified human T cells at concentrations above 20 ng/ml.

Expression system 

E. coli

Sequence 

Recombinant Human Lymphotactin is a 10 kDa protein containing 92 amino acid residues:

GSEVSDKRTC VSLTTQRLPV SRIKTYTITE GSLRAVIFIT KRGLKVCADP QATWVRDVVRSMDRKSNTRN NMIQTKPTGT QQSTNTAVTL TG

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