Overview

  • Product nameAnti-Lyn antibody
    See all Lyn primary antibodies
  • Description
    Rabbit polyclonal to Lyn
  • Tested applicationsWB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    A region between residue 1 and 50 of human Lyn (NP_002341.1)

  • Positive control
    • Ramos (Human Burkitt's lymphoma cell line), 293T lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab95872 was affinity purified using an epitope specific to LYN immobilized on solid support.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab95872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 59 kDa.
IP Use at 5-15 µg/mg of lysate.

Target

  • FunctionDown regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.
  • Tissue specificityWidely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • DomainThe protein kinase domain plays an important role in its localization in the cell membrane.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination is SH3-dependent.
  • Cellular localizationCell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
  • Information by UniProt
  • Database links
  • FormThis protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names
    • AA407514 antibody
    • EC 2.7.10.2 antibody
    • FLJ26625 antibody
    • Hck 2 antibody
    • JTK 8 antibody
    • JTK8 antibody
    • Lck/Yes related novel protein tyrosine kinase antibody
    • LYN antibody
    • LYN_HUMAN antibody
    • ONCOGENE LYN antibody
    • p53Lyn antibody
    • p56Lyn antibody
    • Tyrosine protein kinase LYN antibody
    • Tyrosine-protein kinase Lyn antibody
    • V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody
    • Yamaguchi sarcoma viral (v yes 1) related oncogene homolog antibody
    see all

Anti-Lyn antibody images

  • All lanes : Anti-Lyn antibody (ab95872) at 0.1 µg/ml

    Lane 1 : Ramos cell lysate at 50 µg
    Lane 2 : Ramos cell lysate at 15 µg
    Lane 3 : Ramos cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg


    Predicted band size : 59 kDa
  • Western Blot detection of human Lyn precipitated from Ramos cell lysate using ab95872 at 10µg/mg (1mg/IP; 20% of IP loaded/lane): (1) ab95872 at 1µg/ml; (2) control IgG
    Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-Lyn antibody (ab95872)

ab95872 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"