Overview

  • Product nameAnti-Lyn (phospho Y507) antibody
    See all Lyn primary antibodies
  • Description
    Rabbit polyclonal to Lyn (phospho Y507)
  • Specificityab53122 detects endogenous levels of Lyn only when phosphorylated at tyrosine 507.
  • Tested applicationsIHC, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phosphopeptide derived from human Lyn around the phosphorylation site of tyrosine 507 (G-Q-YP-Q-Q).

  • Positive control
    • K562 cells and human brain tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab53122 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab53122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/300 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • FunctionDown regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.
  • Tissue specificityWidely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • DomainThe protein kinase domain plays an important role in its localization in the cell membrane.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination is SH3-dependent.
  • Cellular localizationCell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
  • Information by UniProt
  • Database links
  • FormThis protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names
    • AA407514 antibody
    • EC 2.7.10.2 antibody
    • FLJ26625 antibody
    • Hck 2 antibody
    • JTK 8 antibody
    • JTK8 antibody
    • Lck/Yes related novel protein tyrosine kinase antibody
    • LYN antibody
    • LYN proto oncogene, Src family tyrosine kinase antibody
    • LYN_HUMAN antibody
    • ONCOGENE LYN antibody
    • p53Lyn antibody
    • p56Lyn antibody
    • Tyrosine protein kinase LYN antibody
    • Tyrosine-protein kinase Lyn antibody
    • V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody
    • Yamaguchi sarcoma viral (v yes 1) related oncogene homolog antibody
    see all

Anti-Lyn (phospho Y507) antibody images

  • All lanes : Anti-Lyn (phospho Y507) antibody (ab53122) at 1/300 dilution

    Lane 1 : K562 cell extract treated with Hydrogen Peroxide (100uM, 30mins)
    Lane 2 : K562 cell extract treated with Hydrogen Peroxide (100uM, 30mins) with immunising peptide


    Predicted band size : 58 kDa
  • ab53122 at 1/50 dilution staining Lyn in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-Lyn (phospho Y507) antibody (ab53122)

This product has been referenced in:
  • Kim S & Kunapuli SP Negative Regulation of Gq-mediated Pathways in Platelets by G12/13 Pathways through Fyn Kinase. J Biol Chem 286:24170-9 (2011). WB ; Human . Read more (PubMed: 21592972) »
  • Gross AJ  et al. Developmental acquisition of the Lyn-CD22-SHP-1 inhibitory pathway promotes B cell tolerance. J Immunol 182:5382-92 (2009). WB ; Mouse . Read more (PubMed: 19380785) »

See all 2 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - saponin
Sample Mouse Cell (B cell line)
Specification B cell line
Gating Strategy Gated on single cells via forward and side light scatter
Preparation Cell harvesting/tissue preparation method: Cells spun down from culture supernatant
Sample buffer: DPBS + 1%FBS + 0.1% azide
Username

Dr. Alan Rosenspire

Verified customer

Submitted Feb 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"