Recombinant Biotin Anti-Lysozyme antibody [EPR2994(2)] (ab197704)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [EPR2994(2)] to Lysozyme
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
Related conjugates and formulations
Overview
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Product name
Biotin Anti-Lysozyme antibody [EPR2994(2)]
See all Lysozyme primary antibodies -
Description
Biotin Rabbit monoclonal [EPR2994(2)] to Lysozyme -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: FFPE human tonsil tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2994(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. -
Involvement in disease
Amyloidosis 8 -
Sequence similarities
Belongs to the glycosyl hydrolase 22 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4069 Human
- Entrez Gene: 17105 Mouse
- Entrez Gene: 17110 Mouse
- Omim: 153450 Human
- SwissProt: P61626 Human
- SwissProt: P08905 Mouse
- Unigene: 524579 Human
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Alternative names
- 1 4 beta N acetylmuramidase C antibody
- 1 antibody
- 4-beta-N-acetylmuramidase C antibody
see all
Images
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IHC image of Lysozyme staining in a section of formalin-fixed paraffin-embedded human tonsil*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab197704, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab197704 has not yet been referenced specifically in any publications.