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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Overview
Product name
Anti-Lysozyme antibody - Lysosome Marker
See all Lysozyme products (16) ...
Description
Rabbit polyclonal to Lysozyme - Lysosome Marker
Specificity
This antibody reacts with lysozyme. It stains granulocytes, monocytes and macrophages in human tonsil, skin and colon. This antibody does not cross-react with any other cell types.
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Lysozyme isolated from urine of patients with monocytic leukemia.
Positive control
In Western Blot, this antibody gave a positive signal in HL60 Whole Cell Lysate and in Human Spleen Tissue Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
unknown
Light chain type
unknown
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Cardiovascular >> Blood >> Other
Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).
IHC-P: 1/50 - 1/100.
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Involvement in disease
Defects in LYZ are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
Sequence similarities
Belongs to the glycosyl hydrolase 22 family.
Cellular localization
Secreted.
Target information above from: UniProt accessionP61626
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1 4 beta n acetylmuramidase c antibody
- 1 antibody
- 4-beta-N-acetylmuramidase C antibody
see all
Database links
- Entrez Gene: 4069 Human
- Omim: 153450 Human
- SwissProt: P61626 Human
- Unigene: 524579 Human
Anti-Lysozyme antibody - Lysosome Marker images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Lysozyme antibody - Lysosome Marker(ab2408)
Ab2408 staining lysozyme in human colon. Staining is localised to cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Western blot - Anti-Lysozyme antibody - Lysosome Marker (ab2408)
All lanes : Anti-Lysozyme antibody - Lysosome Marker (ab2408) at 1 µg/ml
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 17 kDa
Observed band size : 15 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutes
References for Anti-Lysozyme antibody - Lysosome Marker (ab2408)
ab2408 has not yet been referenced specifically in any publications.
Publishing research using ab2408? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"