Anti-Lysozyme antibody - Lysosome Marker (ab391)

I have a customer looking for an alkaline phosphatase conjugated lysozyme antibody, and they specifically asked about AB391. I don't see any alk phos conjugates listed on your web, but they have also asked if you can custom conjugate this antibody if a conjugate is not available. Can you please let me know asap?

Thanks in advance, Jessica

ANSWER:

Thank you for your enquiry. We currently do not have a lysozyme antibody conjugated to AP and we are unable to custom conjugate antibodies.

I would like to perform immunohistochemistry using ab391 on formalin-fixed, paraffin-embedded mouse intestine sections. Do you think your antibody will work for this?

ANSWER:

This antibody has not yet been tested for use with IHC. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher. If you have more questions, please contact us again.

Dear Sir or Madam,

I would like to dectect small amounts of lysozyme by immunoprecipitation and western blot from cell extracts. Have you tested your antibody in these conditions?

Thanks in advance Dr. Manuel Muniz

ANSWER:

Yes, this antibody has been tested by immunoprecipitation and Western blot. The suggested working dilution is 1/4000 to 1/10000, although optimisation in your own lab is recommended.

I wanted to ask about the anti-Lysozyme (product code:ab391, batch 5732) which I recently purchased from you. It appears by Western blotting that although the antibody reacts strongly with egg white lysozyme (detects ca. 0.1ng), there is also reaction with many E. coli proteins. I wanted to ask whether this result could be due to bacterial material in the adjuvant used in immunization? Or is this result unexpected or incorrect? I am using this antibody in conjunction with cloning in E. coli, so that the cross-reaction is a definite problem. Thank you for any information or assistance you can provide.

ANSWER:

Polyclonal antibodies are normally produced in SPF (specific pathogen free) rabbits and these rabbits are free of infection with E. coli. Even with these precautions, quite a lot of polyclonal antibodies give cross-reaction with E. coli proteins. Try using less of the antibody and/or shorter incubation times to reduce the background. If this doesn't help, a good method is to adsorb the antibody with a E. coli protein preparation that does not contain your antigen of interest.

hello sir;

can I use this product for dog antigens in immunoblotting?

thank you

ANSWER:

This antibody is known to work in immunoblotting, however it's reactivity against dog lysozyme has not been determined. As the antibody was raised against lysozyme from chicken egg white, it is uncertain whether it will react with canine.