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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Overview
Product name
Anti-Lysozyme antibody
See all Lysozyme products (16) ...
Description
Rabbit polyclonal to Lysozyme
Specificity
ab34799 recognises purified and partially purified Lysozyme.
Tested applications
Dot Blot, WB, ELISA, IPmore details
Cross reactivity
Reacts with
Chicken
Immunogen
Full length native protein (purified): Lysozyme from Hen Egg White.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.14M Sodium chloride, 0.01M Sodium phosphate. pH 7.4
Concentration
Concentration information loading...
Purification notes
Purified from monospecific antiserum by a delipidation and defibrination.
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Cardiovascular >> Blood >> Other
Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot Blot
ELISA
IP
Application notes
Dot: Use at an assay dependent dilution.
ELISA: 1/1,000 - 1/2,000.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 17 kDa.
This product has been assayed against 1.0 µg of Lysozyme in a standard sandwich ELISA using Peroxidase conjugated Afinity Purified anti-Rabbit IgG and ABTS (2,2'-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid))as a substrate for 30 minutes at room temperature.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Involvement in disease
Defects in LYZ are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
Sequence similarities
Belongs to the glycosyl hydrolase 22 family.
Cellular localization
Secreted.
Target information above from: UniProt accessionP61626
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1 4 beta n acetylmuramidase c antibody
- 1 antibody
- 4-beta-N-acetylmuramidase C antibody
see all
Database links
- Entrez Gene: 396218 Chicken
- SwissProt: P00698 Chicken
References for Anti-Lysozyme antibody (ab34799)
This product has been referenced in:
- Akama Ket al. Protein disulfide isomerase-P5, down-regulated in the final stage of boar epididymal sperm maturation, catalyzes disulfide formation to inhibit protein function in oxidative refolding of reduced denatured lysozyme. Biochim Biophys Acta 1804:1272-84 (2010).Read more (PubMed: 20152940) »
- Setälä NLet al. Interaction of phospholipid transfer protein with human tear fluid mucins. J Lipid Res 51:3126-34 (2010). WB; Human.Read more (PubMed: 20724654) »
See all 2 publications for this product
Publishing research using ab34799? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"