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Product name
Anti-M Cadherin antibody
See all M Cadherin products (5) ...
Description
Rabbit polyclonal to M Cadherin
Specificity
ab65157 detects endogenous levels of total M Cadherin protein.
Tested applications
ICC/IF, ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from an internal sequence of human M Cadherin
Positive control
HepG2 and 293 cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab65157 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - M Cadherin antibody (ab65157)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. PubMed: 20723755
ELISA
ELISA: 1/10000.ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
Target
Relevance
Cadherins are a family of transmembrane glycoproteins that play a key role in Calcium dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal) and M- (muscle) cadherin. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. M-Cadherin is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation.
Cellular localization
Type 1 membrane protein.
Alternative names
- Cadherin 14 antibody
- Cadherin 15 antibody
- Cadherin 3 antibody
see all
Database links
- Entrez Gene: 1013 Human
- Entrez Gene: 12555 Mouse
- Entrez Gene: 361432 Rat
- SwissProt: P55291 Human
- SwissProt: P33146 Mouse
- Unigene: 148090 Human
- Unigene: 1976 Mouse
- Unigene: 18406 Rat
Anti-M Cadherin antibody images:
Western blot - M Cadherin antibody (ab65157)
All lanes : Anti-M Cadherin antibody (ab65157) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : 293 cell extract
Lane 3 : 293 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 90 kDa
Observed band size : 90 kDa
Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
References for Anti-M Cadherin antibody (ab65157)
This product has been referenced in:
- Rezk BM et al. Angiotensin II Infusion Induces Marked Diaphragmatic Skeletal Muscle Atrophy. PLoS One 7:e30276 (2012). WB; Mouse.Read more (PubMed: 22276172) »
- Zhou X et al. Reversal of cancer cachexia and muscle wasting by ActRIIB antagonism leads to prolonged survival. Cell 142:531-43 (2010). ICC/IF; Mouse.Read more (PubMed: 20723755) »
See all 2 publications for this product
Publishing research using ab65157? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"