You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]
See all M13 Bacteriophage Coat Protein g8p products (2) ...

Description

Mouse monoclonal [RL-ph2] to M13 Bacteriophage Coat Protein g8p

Specificity

This antibody reacts with the M13 filamentous phage coat protein g8p.

Tested applications

ICC, Flow Cyt, ELISA, WB, IHC-Frmore details

Immunogen

Isolated M13 phage coat proteins

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Primary antibody notes

The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

Clonality

Monoclonal

Clone number

RL-ph2

Myeloma

Sp2/0-Ag14

Isotype

IgG2a

Light chain type

kappa

Research areas

Microbiology >> Organism >> Virus >> DNA Virus >> single stranded DNA Virus >> Enterobacteria phage M13

  • Western blot - M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)Western blot - M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab9225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

Alternative names

  • Capsid protein G8P antibody
  • Coat protein B antibody
  • Enterobacteria phage M13 coat protein g8p antibody
  • G8P antibody
  • Major coat protein antibody
  • VIII antibody
see all

Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] images:

  Western blot - M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)

Western blot - M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)

Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) at 1/1000 dilution + whole cell lysate prepared from XL10Gold cells at 50 µg

Secondary
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728) at 1/2000 dilution

Predicted band size : 5 kDa
Observed band size : 6 kDa (why is the actual band size different from the predicted?)


Primary antibody incubated for 16 hours at 4°C.
Blocking step performed using 5% milk for 1 hour at room temperature.

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)

This product has been referenced in:

  • Meulemans EVet al. Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody. Hum Antibodies Hybridomas 6:113-8 (1995).Read more (PubMed: 8597626) »
  • Meulemans EVet al. Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody. J Mol Biol 244:353-60 (1994).Read more (PubMed: 7527464) »

See all 2 publications for this product

Publishing research using ab9225? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"