M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)

Product Name 

M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]

Product type 

Primary antibodies

Description 

Mouse monoclonal [RL-ph2] to M13 Bacteriophage Coat Protein g8p

Immunogen 

Isolated M13 phage coat proteins

Reacts with 
(species key)

N/A

Specificity 

This antibody reacts with the M13 filamentous phage coat protein g8p.

Tested applications 
(see key)

ELISA, Flow Cyt, ICC, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-Fr: Recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
WB: 1/100 - 1/1000.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Microbiology >> Organism >> Virus >> DNA Virus >> single stranded DNA Virus >> Enterobacteria phage M13

Relevance 

M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells.
Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities.
Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated.
The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest.
Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

RL-ph2

Myeloma 

Sp2/0-Ag14

Isotype 

IgG2a

Light chain type 

kappa

Purity 

Protein A purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)

  This product has been used in: (two most recent references)

Meulemans EV et al. Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody. Hum Antibodies Hybridomas 6:113-8 (1995). PubMed: 8597626

Meulemans EV et al. Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody. J Mol Biol 244:353-60 (1994). PubMed: 7527464
If you publish research using ab9225 please let us know so that we can cite the reference on this datasheet.

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