Anti-M13 + Fd Bacteriophage Coat Proteins antibody (ab6188)

I would like to get more vials of lot 861626. How many more are available?

ANSWER:

Unfortunately lot 861626 is an individual batch which was not produced from a parent batch, also I'm afraid that we no longer have stock of this batch in Europe or the USA. Sorry that I don't have better news; if you have any further questions please do not hesitate to contact me.

 Hi, I am wondering if peptide mimotope of your Anti-M13 + Fd Bacteriophage Coat Proteins antibody is known?

ANSWER:

Thank you very much for your inquiry. The immunogen for this polyclonal antibody was a cell preparation of fd bacteriophage. We therefore do not know what epitope is bound exactly by this antibody. Therefore we also cannot make any predictions about the possible mimotopes (A mimotope is a macromolecule which mimics the structure of an epitope. Because of this property it causes an antibody response identical to the one elicited by the epitope.) that are recognized by this antibody. I am sorry I could not be of more help on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me again with any further questions.

Thank you very much for reply. So do you supply the anti-pVIII protein of M13 phage antibody for ELISA (used in phage display experiment)? And HRP/AntiM13 and HRP substrate OPD (O-phenyldiamine dihydrochloride)?

Thank you very much.

ANSWER:

Thank you for contacting us.

We have two anti-M13 antibodies that may be of interest:

ab6188 Anti-M13 + Fd Bacteriophage Coat Proteins antibody

Binds specifically to phage coat proteins of M13 or fd phage.

Click here (or use the following: http://www.abcam.com/index.html?datasheet=6188).

ab50370 Anti-M13 antibody [B62-FE2] (HRP)

Binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT.

Click here (or use the following: http://www.abcam.com/index.html?datasheet=50370).

We have a number of HRP kits, mainly for IHC. Here is a link to a HRP substrate than can be used for WB:

ab5801 Chemiluminescent HRP Substrate (membrane)

Click here (or use the following: http://www.abcam.com/index.html?datasheet=5801).

Please let me know if there is anything else I can help you with.

Do you have antibodies for phage proteins?

ANSWER:

Thank you for your reply.

We have a number of antibodies to Bacteriophage Proteins such as ab6188: Anti-M13 + Fd Bacteriophage Coat Proteins antibody or ab9225: Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2].

I hope this helps, please let me know if you need any additional information.

I have a question regarding antibody ab6188 (M13 bacteriophage coat proteins). We used this antibody in immunofluorescence stainings and observed an intense cross-reactivity with (non M13-infected) E. coli, apparently with one of the outer membrane proteins. In fact, it was the major signal (see attached pictures). We used the antibody in a 1:50 dilution in PBS and used skim milk powder as blocking reagent. Detection was with an Alexa Fluor 488 - coupled secondary antibody. A negative control (ab6188 omitted) resulted in zero fluorescence, as expected. As we want to use the antibody in part to discriminate between infected and non-infected E. coli, this is clearly unacceptable. Do you have any suggestion how to solve this problem?

ANSWER:

Thank you for your enquiry.

I have been in correspondence with the source of this antibody. Following discussion we concluded that there may be a degree of contamination in your samples. There is clear specific reactivity with your M13- cells. Can you tell me whether it is a possibility that this may have happened? This is supported by the fact that there is variable fluorescence in the cells, possibly reflective of the variable copy number in the M13 infection. I consider it unlikely that this antibody is demonstrating reactivity with non-specific E.coli proteins.

I appreciate your comments.