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Products:Microbiology >> Organism >> Virus >> DNA Virus >> single stranded DNA Virus >> Enterobacteria phage M13
Overview
Product name
Anti-M13 + Fd Bacteriophage Coat Proteins antibody
Description
Rabbit polyclonal to M13 + Fd Bacteriophage Coat Proteins
Specificity
Binds specifically to phage coat proteins of M13 or fd phage.
Tested applications
Indirect ELISA, ELISA, IFmore details
Immunogen
fd bacteriophage
General notes
Concentration: 5.5 mg/ml- 7.5 mg/ml
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.
Clonality
Polyclonal
Isotype
IgG
Research areas
Microbiology >> Organism >> Virus >> DNA Virus >> single stranded DNA Virus >> Enterobacteria phage M13
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
I-ELISA: Use at an assay dependent dilution. (At least 1/1000 for 5 x107 phage/mlat least 1/8000 for 5 x 1010 phage/ml.)
ELISA: Use at an assay dependent concentration.
IF: Use at an assay dependent dilution.
Target
Relevance
M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends.
Alternative names
- Coat protein A antibody
- Coat protein B antibody
- Coat protein C polypeptide I antibody
see all
References for Anti-M13 + Fd Bacteriophage Coat Proteins antibody (ab6188)
This product has been referenced in:
- Wu Det al. Phage Displayed Peptides to Avian H5N1 Virus Distinguished the Virus from Other Viruses. PLoS One 6:e23058 (2011).Read more (PubMed: 21887228) »
- Antonara Set al. Borrelia burgdorferi adhesins identified using in vivo phage display. Mol Microbiol 66:262-76 (2007). ELISA.Read more (PubMed: 17784908) »
See all 5 publications for this product
Publishing research using ab6188? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"