M13 + Fd Bacteriophage Coat Proteins antibody (ab6188)

Product Name 

M13 + Fd Bacteriophage Coat Proteins antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to M13 + Fd Bacteriophage Coat Proteins

Immunogen 

fd bacteriophage

Specificity 

Binds specifically to phage coat proteins of M13 or fd phage.

Tested applications 
(see key)

I-ELISA, ICC/IF

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IF: Use at an assay dependant dilution.
I-ELISA: at least 1/1000 for 5 x10⁷ phage/ml
at least 1/8000 for 5 x 10¹⁰ phage/ml

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Microbiology >> Organism >> Virus >> DNA Virus >> single stranded DNA Virus >> Enterobacteria phage M13

Relevance 

M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends.

Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage.
May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

0.01M PBS pH7.4, 15mM Na azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 3.60 - 3.70 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Concentration: 5.5 mg/ml- 7.5 mg/ml

References for M13 + Fd Bacteriophage Coat Proteins antibody (ab6188)

  This product has been used in: (two most recent references)

Antonara S et al. Borrelia burgdorferi adhesins identified using in vivo phage display. Mol Microbiol 66:262-76 (2007). ELISA. PubMed: 17784908

Hekim C et al. Novel peptide inhibitors of human kallikrein 2. J Biol Chem 281:12555-60 (2006). PubMed: 16527822

See all 3 publication references for this product.


If you publish research using ab6188 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding M13 + Fd Bacteriophage Coat Proteins antibody

Customer FAQs regarding M13 + Fd Bacteriophage Coat Proteins antibody

Protocols for M13 + Fd Bacteriophage Coat Proteins antibody

Price and availability of products related to M13 + Fd Bacteriophage Coat Proteins antibody

M13 + Fd Bacteriophage Coat Proteins antibody - related products: