Overview
- Product nameAnti-M13 antibody [E1] (Biotin)See all M13 primary antibodies ...
- DescriptionMouse monoclonal [E1] to M13 (Biotin)
- ConjugationBiotin
- SpecificityM13 antibody [E1] (ab17269) is specific for gp3 protein.
- Tested applicationsWB, ELISA, Flow Cyt more details
- Species reactivityM13 antibody [E1] (ab17269) is non-species specific. Not yet tested in other species.
- Immunogen
Full length M13 phage coat protein; Exact epitope not determined.
- General notes
M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.08% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityProtein A purified
- Purification notesM13 antibody [E1] (ab17269) is purified using 0.2µm sterile filter.
- Primary antibody notes M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
- Clonality Monoclonal
- Clone numberE1
- Myelomaunknown
- IsotypeIgG2a
- Light chain typeunknown
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab17269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use at an assay dependent dilution. Detects a band of approximately 90 kDa. |
| ELISA | ELISA: Use at an assay dependent dilution. |
| Flow Cyt | Flow Cyt: Use at an assay dependent dilution. |
Target
- RelevanceThe bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.
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Alternative names
- Bacteriophage M13 antibodyColiphage M13 antibodyEnterobacteria phage M13 antibody
- M13 virus antibodyPhage M13 antibody
see all
References for Anti-M13 antibody [E1] (Biotin) (ab17269)
ab17269 has not yet been referenced specifically in any publications.
