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Products:Signal Transduction >> Protein Trafficking >> Golgi Proteins
Overview
Product name
Anti-M6PRBP1 antibody
See all M6PRBP1 products (7) ...
Description
Rabbit polyclonal to M6PRBP1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
A 14 amino acid peptide from near the amino terminus of human M6PRBP1.
Positive control
EL4 cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
IHC-P: Use a concentration of 2 µg/ml
Target
Function
Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
Sequence similarities
Belongs to the perilipin family.
Cellular localization
Cytoplasm. Endosome membrane. Lipid droplet. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
Target information above from: UniProt accessionO60664
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 47 kDa MPR binding protein antibody
- Cargo selection protein TIP47 antibody
- 47 kDa mannose 6-phosphate receptor-binding protein antibody
see all
Database links
- Entrez Gene: 10226 Human
- Entrez Gene: 66905 Mouse
- Entrez Gene: 316130 Rat
- NCBI: NP_005808.2 Human
- Omim: 602702 Human
- SwissProt: O60664 Human
- SwissProt: Q2XSQ4 Mouse
- SwissProt: Q9DBG5 Mouse
see all
Anti-M6PRBP1 antibody images:
Western blot - M6PRBP1 antibody (ab47639)
Lane 1 : Anti-M6PRBP1 antibody (ab47639) at 0.5 µg/ml
Lane 2 : Anti-M6PRBP1 antibody (ab47639) at 1 µg/ml
Lane 1 : EL4 cells
Lane 2 : EL4 cells
Lysates/proteins at 15 µg per lane.
Secondary
Anti-rabbit IgG HRP at 1:10000
Predicted band size : 48 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - M6PRBP1 antibody (ab47639)
ab47639 at 2µg/ml staining M6PRBP1 in rat liver tissue by IHC
References for Anti-M6PRBP1 antibody (ab47639)
This product has been referenced in:
- García-Faroldi Get al. Polyamines are present in mast cell secretory granules and are important for granule homeostasis. PLoS One 5:e15071 (2010). WB; Mouse.Read more (PubMed: 21151498) »
See 1 publication for this product
Publishing research using ab47639? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"