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Anti-MAD1 antibody [MAD1D6H4] (ab50791)

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    Overview

    Product name

    Anti-MAD1 antibody [MAD1D6H4]
    See all MAD1 products (5) ...

    Description

    Mouse monoclonal [MAD1D6H4] to MAD1

    Tested applications

    WBmore details

    Cross reactivity

    Reacts with

    Human

    Immunogen

    Recombinant Human MAD1

    Properties

    Form

    Liquid

    Storage instructions

    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

    Storage buffer

    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4

    Concentration

    Concentration information loading...

    Purity

    Protein G purified

    Purification notes

    The antibody was filtered through an 0.22 µm membrane.

    Clonality

    Monoclonal

    Clone number

    MAD1D6H4

    Isotype

    IgG2b

    Research areas

    Cell Biology >> Cell Cycle >> Cell Division >> Spindle

    • Western blot - MAD1 antibody [MAD1D6H4] (ab50791)Western blot - MAD1 antibody [MAD1D6H4] (ab50791) image (enlarge)

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab50791 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Target

    Relevance

    MAD1 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, MAD1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad1p becomes hyperphosphorylated upon spindle depolymerization.

    Cellular localization

    Nuclear

    Alternative names

    • hMAD1 antibody
    • HsMAD1 antibody
    • MAD1L1 antibody
    • Mitotic arrest deficient 1 antibody
    • Mitotic checkpoint MAD1 protein homolog antibody
    • Mitotic spindle assembly checkpoint protein MAD1 antibody
    • PIG9 antibody
    • Tax binding protein 181 antibody
    • TP53I9 antibody
    • Tumor protein p53 inducible protein 9 antibody
    • TXBP181 antibody
    see all

    Database links

    Anti-MAD1 antibody [MAD1D6H4] images:

      Western blot - MAD1 antibody [MAD1D6H4] (ab50791)

    Western blot - MAD1 antibody [MAD1D6H4] (ab50791)

    Anti-MAD1 antibody [MAD1D6H4] (ab50791) + Immunised recombinant protein

    Predicted band size : 83 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    References for Anti-MAD1 antibody [MAD1D6H4] (ab50791)

    ab50791 has not yet been referenced specifically in any publications.

    Publishing research using ab50791? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"