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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-MAD1 antibody
See all MAD1 products (5) ...
Description
Rabbit polyclonal to MAD1
Tested applications
WB, IFmore details
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Recombinant fusion protein (S. cerevisiae).
Positive control
S.cerevisiae whole cell lysate (see images).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: Whole Serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100Detects a band of approximately 83 kDa.
IF
IF: 1/100IF: 1/100
Target
Relevance
MAD1 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, MAD1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad1p becomes hyperphosphorylated upon spindle depolymerization.
Cellular localization
Nuclear
Alternative names
- hMAD1 antibody
- HsMAD1 antibody
- MAD1L1 antibody
see all
Database links
- Entrez Gene: 852794 Saccharomyces cerevisiae
- SwissProt: P40957 Saccharomyces cerevisiae
Anti-MAD1 antibody images:
Western blot - MAD1 antibody (ab11691)
All lanes : Anti-MAD1 antibody (ab11691) at 1/100 dilution
Lane 1 : Wild type S. cerevisiae whole cell lysate
Lane 2 : mad1 knockout S. cerevisiae whole cell lysate
developed using the ECL technique
Observed band size : 83 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - MAD1 antibody (ab11691)
ab11691 at a 1/100 dilution staining MAD1 in S.cerevisiae by immunofluorescence.
References for Anti-MAD1 antibody (ab11691)
ab11691 has not yet been referenced specifically in any publications.
Publishing research using ab11691? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"