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Anti-MAD1 antibody (ab45286)

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Overview

Product name

Anti-MAD1 antibody
See all MAD1 products (5) ...

Description

Rabbit polyclonal to MAD1

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Recombinant MAD1 protein fragment (Human).

Positive control

MCF-7, T47D and MDA435 cell lysates.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - MAD1 antibody (ab45286)Western blot - MAD1 antibody (ab45286) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab45286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

MAD1 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, MAD1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad1p becomes hyperphosphorylated upon spindle depolymerization.

Cellular localization

Nuclear

Alternative names

  • hMAD1 antibody
  • HsMAD1 antibody
  • MAD1L1 antibody
  • Mitotic arrest deficient 1 antibody
  • Mitotic arrest deficient 1 antibody
  • Mitotic checkpoint MAD1 protein homolog antibody
  • Mitotic spindle assembly checkpoint protein MAD1 antibody
  • PIG9 antibody
  • Tax binding protein 181 antibody
  • TP53I9 antibody
  • Tumor protein p53 inducible protein 9 antibody
  • TXBP181 antibody
see all

Anti-MAD1 antibody images:

  Western blot - MAD1 antibody (ab45286)

Western blot - MAD1 antibody (ab45286)

All lanes : Anti-MAD1 antibody (ab45286) at 1/2000 dilution

Lane 1 : MCF-7 whole cell lysate (50µg)
Lane 2 : T47D whole cell lysate (50µg)
Lane 3 : MDA435 whole cell lysate (50µg)


Predicted band size : 80 kDa

References for Anti-MAD1 antibody (ab45286)

ab45286 has not yet been referenced specifically in any publications.

Publishing research using ab45286? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"