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Western blot - MAD1 antibody (ab45286)
(enlarge)
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Product Name
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MAD1 antibody
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See all MAD1 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MAD1
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Immunogen
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Recombinant MAD1 protein fragment (Human).
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/2000. Predicted molecular weight: 80 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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MCF-7, T47D and MDA435 cell lysates.
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Cellular localization
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Nuclear
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Research areas
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Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Relevance
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MAD1 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, MAD1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad1p becomes hyperphosphorylated upon spindle depolymerization.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAD1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAD1 antibody images, references, products related to ab45286 and other tools.
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MAD1 antibody images:
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 Western blot - MAD1 antibody (ab45286)
All lanes : MAD1 antibody (ab45286) at 1/2000 dilution
Lane 1 : MCF-7 whole cell lysate (50µg)
Lane 2 : T47D whole cell lysate (50µg)
Lane 3 : MDA435 whole cell lysate (50µg)
Predicted band size : 80 kDa
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Search PubMed (MEDLINE) for references to MAD1
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MAD1 antibody - more information
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Customer reviews (feedback) regarding MAD1 antibody |
Customer FAQs regarding MAD1 antibody |
Protocols for MAD1 antibody |
Price and availability of products related to MAD1 antibody |
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MAD1 antibody - related products:
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MAD1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MAD1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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