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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-MAD1 antibody
See all MAD1 products (5) ...
Description
Rabbit polyclonal to MAD1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Recombinant MAD1 protein fragment (Human).
Positive control
MCF-7, T47D and MDA435 cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Western blot - MAD1 antibody (ab45286)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Predicted molecular weight: 80 kDa.
Target
Relevance
MAD1 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, MAD1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad1p becomes hyperphosphorylated upon spindle depolymerization.
Cellular localization
Nuclear
Alternative names
- hMAD1 antibody
- HsMAD1 antibody
- MAD1L1 antibody
see all
Database links
- Entrez Gene: 8379 Human
- Entrez Gene: 17120 Mouse
- Omim: 602686 Human
- SwissProt: Q9Y6D9 Human
- SwissProt: P50538 Mouse
- SwissProt: Q9WTX8 Mouse
- Unigene: 209128 Human
Anti-MAD1 antibody images:
Western blot - MAD1 antibody (ab45286)
All lanes : Anti-MAD1 antibody (ab45286) at 1/2000 dilution
Lane 1 : MCF-7 whole cell lysate (50µg)
Lane 2 : T47D whole cell lysate (50µg)
Lane 3 : MDA435 whole cell lysate (50µg)
Predicted band size : 80 kDa
References for Anti-MAD1 antibody (ab45286)
ab45286 has not yet been referenced specifically in any publications.
Publishing research using ab45286? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"