![Western blot - Anti-MAD1 antibody [9B10] (ab5783) image](#ab5783_1.jpg)
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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-MAD1 antibody [9B10]
See all MAD1 products (5) ...
Description
Mouse monoclonal [9B10] to MAD1
Tested applications
IP, IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length MAD1 protein (Human)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
9B10
Isotype
IgG2b
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Western blot - Anti-MAD1 antibody [9B10] (ab5783)
![Western blot - Anti-MAD1 antibody [9B10] (ab5783) image](/ps/datasheet/Images/5/ab5783/ab5783_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab5783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
IF
Application notes
IF: 1/500 - 1/1000.
IP: Use at a concentration of 5 µg/0.5 - 1ml of extract.
This antibody has currently been unsuccessful in WB.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
MAD1 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, MAD1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad1p becomes hyperphosphorylated upon spindle depolymerization.
Cellular localization
Nuclear
Alternative names
- hMAD1 antibody
- HsMAD1 antibody
- MAD-1 antibody
see all
Database links
- Entrez Gene: 8379 Human
- Omim: 602686 Human
- SwissProt: Q9Y6D9 Human
- Unigene: 209128 Human
Anti-MAD1 antibody [9B10] images:
Western blot - Anti-MAD1 antibody [9B10] (ab5783)
Predicted band size : 96 kDa
0.5ml of HeLa cell lysate was incubated with 5µg of ab5783 and the immunecomplex immunoprecipitated with protein G coupled beads. The post-immunoprecipitate (lane 3), the immunoprecipitate (lane 2) and whole HeLa cel lysate (lane 1) was transfered to the membrane and probed with rabbit anti-MAD1.
MAD1 is shown as an approximately 96 kDa band. The lower molecular weight bands are degredation products.
References for Anti-MAD1 antibody [9B10] (ab5783)
ab5783 has not yet been referenced specifically in any publications.
Publishing research using ab5783? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"