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- MAD1 antibody [9B10] (ab5783)
![- MAD1 antibody [9B10] (ab5783)](/ps/datasheet/images/ab5783_1.jpg)
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Product Name
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MAD1 antibody [9B10]
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See all MAD1 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [9B10] to MAD1
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Immunogen
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Recombinant full length MAD1 protein (Human)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF: 1/500 - 1/1000.
IP: Use at a concentration of 5 µg/0.5 - 1ml of extract.
This antibody has currently been unsuccessful in WB.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Relevance
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MAD1 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, MAD1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad1p becomes hyperphosphorylated upon spindle depolymerization.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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9B10
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Isotype
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IgG2b
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAD1 antibody [9B10] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAD1 antibody [9B10] images, references, products related to ab5783 and other tools.
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MAD1 antibody [9B10] images:
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 - MAD1 antibody [9B10] (ab5783)
Predicted band size : 96 kDa
0.5ml of HeLa cell lysate was incubated with 5µg of ab5783 and the immunecomplex immunoprecipitated with protein G coupled beads. The post-immunoprecipitate (lane 3), the immunoprecipitate (lane 2) and whole HeLa cel lysate (lane 1) was transfered to the membrane and probed with rabbit anti-MAD1.
MAD1 is shown as an approximately 96 kDa band. The lower molecular weight bands are degredation products.
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Search PubMed (MEDLINE) for references to MAD1
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MAD1 antibody [9B10] - more information
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Customer reviews (feedback) regarding MAD1 antibody [9B10] |
Customer FAQs regarding MAD1 antibody [9B10] |
Protocols for MAD1 antibody [9B10] |
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MAD1 antibody [9B10] - related products:
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MAD1 antibody [9B10] - other tools:
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Contact Abcam with a Technical Enquiry about MAD1 antibody [9B10] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![- MAD1 antibody [9B10] (ab5783)](/index.html?datasheet=5783#ab5783_1.jpg)
