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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-MAD2 antibody
See all MAD2 products (4) ...
Description
Rabbit polyclonal to MAD2
Tested applications
WB, IFmore details
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Recombinant bacterial His6-fusion protein (S. cerevisiae).
Positive control
S.cerevisiae nuclear extracts (see image below).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: Whole Serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Western blot - MAD2 antibody (ab11690)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100Detects a band of approximately 23 kDa.
IF
IF: 1/100IF: 1/100
Target
Relevance
MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. hMAD2 is the human homologue of yeast MAD2.
Cellular localization
Nuclear
Alternative names
- HSMAD2 antibody
- Human mitotic feedback control protein Madp2 homolog mRNA complete cds antibody
- MAD 2 antibody
see all
Anti-MAD2 antibody images:
Western blot - MAD2 antibody (ab11690)
ab11690 at a 1/100 dilution staining approximately 23kDa S.cerevisiae MAD2 in nucleoplasm extract, nuclear membrane extract and whole nuclear extract by Western blot (ECL).
References for Anti-MAD2 antibody (ab11690)
This product has been referenced in:
- Tang Jet al. Phosphorylation of Plk1 at Ser326 regulates its functions during mitotic progression. Oncogene 27:6635-45 (2008).Read more (PubMed: 18695677) »
See 1 publication for this product
Publishing research using ab11690? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"