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Western blot - MAD2 antibody (ab11690)
(enlarge)
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Product Name
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MAD2 antibody
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See all MAD2 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MAD2
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Immunogen
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Recombinant bacterial His6-fusion protein (S. cerevisiae).
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Reacts with
(species key)
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Sc
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF: 1/100.
WB: 1/100. Detects a band of approximately 23 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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S.cerevisiae nuclear extracts (see image below).
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Relevance
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MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators.
hMAD2 is the human homologue of yeast MAD2.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Whole Serum Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAD2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAD2 antibody images, references, products related to ab11690 and other tools.
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MAD2 antibody images:
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 Western blot - MAD2 antibody (ab11690)
ab11690 at a 1/100 dilution staining approximately 23kDa S.cerevisiae MAD2 in nucleoplasm extract, nuclear membrane extract and whole nuclear extract by Western blot (ECL).
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References for MAD2 antibody (ab11690)
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This product has been used in:
Tang J et al. Phosphorylation of Plk1 at Ser326 regulates its functions during mitotic progression. Oncogene 27:6635-45 (2008). PubMed: 18695677
If you publish research using ab11690 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MAD2
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MAD2 antibody - more information
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Customer reviews (feedback) regarding MAD2 antibody |
Customer FAQs regarding MAD2 antibody |
Protocols for MAD2 antibody |
Price and availability of products related to MAD2 antibody |
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MAD2 antibody - related products:
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MAD2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MAD2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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