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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-MAD2 antibody
See all MAD2 products (4) ...
Description
Rabbit polyclonal to MAD2
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human MAD2.
(Peptide available as ab61590.)
Positive control
This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa HepG2
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Western blot - MAD2 antibody (ab61591)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Target
Relevance
MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. hMAD2 is the human homologue of yeast MAD2.
Cellular localization
Nuclear
Alternative names
- HSMAD2 antibody
- Human mitotic feedback control protein Madp2 homolog mRNA complete cds antibody
- MAD 2 antibody
see all
Database links
- Entrez Gene: 4085 Human
- Entrez Gene: 56150 Mouse
- Omim: 601467 Human
- SwissProt: Q13257 Human
- SwissProt: Q9Z1B5 Mouse
- Unigene: 591697 Human
- Unigene: 290830 Mouse
Anti-MAD2 antibody images:
Western blot - MAD2 antibody (ab61591)
All lanes : Anti-MAD2 antibody (ab61591) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 24 kDa
Observed band size : 24 kDa
References for Anti-MAD2 antibody (ab61591)
ab61591 has not yet been referenced specifically in any publications.
Publishing research using ab61591? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"