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Western blot - MAD2 antibody (ab70383)
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Product Name
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MAD2 antibody
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See all MAD2 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MAD2
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Immunogen
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Synthetic peptide mapping to a region between residues 100 and 150 of human MAD2 (according to SwissProt entry Q13257)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2-4µg/mg of lysate.
WB: 1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa nuclear lysate
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Relevance
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MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators.
hMAD2 is the human homologue of yeast MAD2.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAD2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAD2 antibody images, references, products related to ab70383 and other tools.
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MAD2 antibody images:
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 Western blot - MAD2 antibody (ab70383)
MAD2 antibody (ab70383) at 1 µg/ml + NETN whole cell lysate from HeLa cells at 50 µg
developed using the ECL technique
Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
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References for MAD2 antibody (ab70383)
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This product has been used in: (two most recent references)
Agoston AT et al. Retinoblastoma pathway dysregulation causes DNA methyltransferase 1 overexpression in cancer via MAD2-mediated inhibition of the anaphase-promoting complex. Am J Pathol 170:1585-93 (2007). WB; Human. PubMed: 17456764
Vader G et al. The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule kinetochore interactions. Mol Biol Cell 18:4553-64 (2007). Human. PubMed: 17699588
See all 3 publication references for this product.
If you publish research using ab70383 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MAD2
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MAD2 antibody - more information
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Customer reviews (feedback) regarding MAD2 antibody |
Customer FAQs regarding MAD2 antibody |
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Price and availability of products related to MAD2 antibody |
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MAD2 antibody - related products:
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MAD2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MAD2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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