Overview

  • Product nameAnti-MAD2 antibody
    See all MAD2 primary antibodies
  • Description
    Rabbit polyclonal to MAD2
  • Tested applicationsWB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide mapping to a region between residue 150 and the C-terminus (residue 205) of Human MAD2 (SwissProt Q13257)

  • Positive control
    • NETN whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2 - 4 µg/mg of lysate.
    WB: 1/5000 - 1/20000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
    • Sequence similaritiesBelongs to the MAD2 family.
      Contains 1 HORMA domain.
    • DomainThe protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
    • Post-translational
      modifications
      Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Phosphorylated by NEK2.
    • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.
    • Information by UniProt
    • Database links
    • Alternative names
      • HSMAD2 antibody
      • Human mitotic feedback control protein Madp2 homolog mRNA complete cds antibody
      • MAD 2 antibody
      • MAD2 like 1 antibody
      • MAD2 mitotic arrest deficient like 1 antibody
      • MAD2-like protein 1 antibody
      • MAD2B antibody
      • MAD2L1 antibody
      • MD2L1_HUMAN antibody
      • Mitotic arrest deficient 2 antibody
      • Mitotic arrest deficient 2-like protein 1 antibody
      • Mitotic spindle assembly checkpoint protein MAD2A antibody
      • REV7 antibody
      see all

    Anti-MAD2 antibody images

    • Anti-MAD2 antibody (ab70385) at 1/5000 dilution + NETN whole cell lysate from HeLa cells at 50 µg
      developed using the ECL technique

      Predicted band size : 24 kDa
      Observed band size : 24 kDa
      Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute
    • Detection of Human MAD2 by Immunoprecipitation in NETN whole cell lysate from HeLa cells (600 µg/IP) using ab70385 at 0.7 µg/mg for IP and at 0.2 µg/ml for subsequent WB detection. Note: bands between 50 and 60 kDa represent the IgG chains.

    References for Anti-MAD2 antibody (ab70385)

    ab70385 has not yet been referenced specifically in any publications.

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