Overview

  • Product nameAnti-MAD2 antibodySee all MAD2 primary antibodies ...
  • Description
    Rabbit polyclonal to MAD2
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide mapping to a region between residue 150 and the C-terminus (residue 205) of Human MAD2 (SwissProt Q13257)

  • Positive control
    • NETN whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
  • Application notesIP: Use at 2 - 4 µg/mg of lysate.
    WB: 1/5000 - 1/20000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. hMAD2 is the human homologue of yeast MAD2.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • HSMAD2 antibody
      • Human mitotic feedback control protein Madp2 homolog mRNA complete cds antibody
      • MAD 2 antibody
      • MAD2 like 1 antibody
      • MAD2 mitotic arrest deficient like 1 antibody
      • MAD2B antibody
      • MAD2L1 antibody
      • Mitotic arrest deficient 2 antibody
      • Mitotic spindle assembly checkpoint protein MAD2A antibody
      • REV7 antibody
      see all

    Anti-MAD2 antibody images

    • Anti-MAD2 antibody (ab70385) at 1/5000 dilution + NETN whole cell lysate from HeLa cells at 50 µg
      developed using the ECL technique

      Predicted band size : 24 kDa
      Observed band size : 24 kDa
      Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute
    • Detection of Human MAD2 by Immunoprecipitation in NETN whole cell lysate from HeLa cells (600 µg/IP) using ab70385 at 0.7 µg/mg for IP and at 0.2 µg/ml for subsequent WB detection. Note: bands between 50 and 60 kDa represent the IgG chains.

    References for Anti-MAD2 antibody (ab70385)

    ab70385 has not yet been referenced specifically in any publications.

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