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Read our guarantee »Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Anti-MAD2 antibody
See all MAD2 products (4) ...
Rabbit polyclonal to MAD2
WB, IPmore details
Reacts with
Human
Predicted to work with
Mouse
Synthetic peptide mapping to a region between residue 150 and the C-terminus (residue 205) of Human MAD2 (SwissProt Q13257)
NETN whole cell lysate from HeLa cells.
Liquid
Store at +4°C.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7.0-8.0
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Immunogen affinity purified
Polyclonal
IgG
Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Our Abpromise guarantee covers the use of ab70385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2 - 4 µg/mg of lysate.
WB: 1/5000 - 1/20000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. hMAD2 is the human homologue of yeast MAD2.
Nuclear
Western blot - MAD2 antibody (ab70385)

Anti-MAD2 antibody (ab70385) at 1/5000 dilution + NETN whole cell lysate from HeLa cells at 50 µg
developed using the ECL technique
Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
Immunoprecipitation - MAD2 antibody (ab70385)

Detection of Human MAD2 by Immunoprecipitation in NETN whole cell lysate from HeLa cells (600 µg/IP) using ab70385 at 0.7 µg/mg for IP and at 0.2 µg/ml for subsequent WB detection. Note: bands between 50 and 60 kDa represent the IgG chains.
ab70385 has not yet been referenced specifically in any publications.
Publishing research using ab70385? Please let us know so that we can cite the reference in this datasheet
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Anti-MAD2 antibody (ab70385) at 1/5000 dilution + NETN whole cell lysate from HeLa cells at 50 µg
developed using the ECL technique
Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute

Detection of Human MAD2 by Immunoprecipitation in NETN whole cell lysate from HeLa cells (600 µg/IP) using ab70385 at 0.7 µg/mg for IP and at 0.2 µg/ml for subsequent WB detection. Note: bands between 50 and 60 kDa represent the IgG chains.
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