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Overview

  • Product nameAnti-MAD2 antibodySee all MAD2 primary antibodies ...
  • Description
    Rabbit polyclonal to MAD2
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide mapping to a region between residue 150 and the C-terminus (residue 205) of Human MAD2 (SwissProt Q13257)

  • Positive controlNETN whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
  • Application notesIP: Use at 2 - 4 µg/mg of lysate.
    WB: 1/5000 - 1/20000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceMAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. hMAD2 is the human homologue of yeast MAD2.
    • Cellular localizationNuclear
    • Database links
      • Alternative names
          HSMAD2 antibodyHuman mitotic feedback control protein Madp2 homolog mRNA complete cds antibodyMAD 2 antibody
          MAD2 like 1 antibodyMAD2 mitotic arrest deficient like 1 antibodyMAD2B antibodyMAD2L1 antibodyMitotic arrest deficient 2 antibodyMitotic spindle assembly checkpoint protein MAD2A antibodyREV7 antibody
        see all

      Anti-MAD2 antibody images

      • Western blot - MAD2 antibody (ab70385)
        Western blot - MAD2 antibody (ab70385)
        Anti-MAD2 antibody (ab70385) at 1/5000 dilution + NETN whole cell lysate from HeLa cells at 50 µg
        developed using the ECL technique

        Predicted band size : 24 kDa
        Observed band size : 24 kDa
        Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

        Exposure time : 1 minute
      • Immunoprecipitation - MAD2 antibody (ab70385)
        Immunoprecipitation - MAD2 antibody (ab70385)
        Detection of Human MAD2 by Immunoprecipitation in NETN whole cell lysate from HeLa cells (600 µg/IP) using ab70385 at 0.7 µg/mg for IP and at 0.2 µg/ml for subsequent WB detection. Note: bands between 50 and 60 kDa represent the IgG chains.

      References for Anti-MAD2 antibody (ab70385)

      ab70385 has not yet been referenced specifically in any publications.

      Publishing research using ab70385 ? Please let us know so that we can cite the reference in this datasheet

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