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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-MAD2 antibody
See all MAD2 products (4) ...
Description
Rabbit polyclonal to MAD2
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide mapping to a region between residue 150 and the C-terminus (residue 205) of Human MAD2 (SwissProt Q13257)
Positive control
NETN whole cell lysate from HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7.0-8.0
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at 2 - 4 µg/mg of lysate.
WB: 1/5000 - 1/20000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. hMAD2 is the human homologue of yeast MAD2.
Cellular localization
Nuclear
Alternative names
- HSMAD2 antibody
- Human mitotic feedback control protein Madp2 homolog mRNA complete cds antibody
- MAD 2 antibody
see all
Database links
- Entrez Gene: 4085 Human
- Entrez Gene: 56150 Mouse
- Omim: 601467 Human
- SwissProt: Q13257 Human
- SwissProt: Q9Z1B5 Mouse
- Unigene: 591697 Human
- Unigene: 290830 Mouse
Anti-MAD2 antibody images:
Western blot - MAD2 antibody (ab70385)
Anti-MAD2 antibody (ab70385) at 1/5000 dilution + NETN whole cell lysate from HeLa cells at 50 µg
developed using the ECL technique
Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
Immunoprecipitation - MAD2 antibody (ab70385)
Detection of Human MAD2 by Immunoprecipitation in NETN whole cell lysate from HeLa cells (600 µg/IP) using ab70385 at 0.7 µg/mg for IP and at 0.2 µg/ml for subsequent WB detection. Note: bands between 50 and 60 kDa represent the IgG chains.
References for Anti-MAD2 antibody (ab70385)
ab70385 has not yet been referenced specifically in any publications.
Publishing research using ab70385? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"