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MitoSciences (MS782)

Anti-ETFA antibody [2B11AE8] (ab110316)

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Overview

  • Product nameAnti-ETFA antibody [2B11AE8]See all ETFA primary antibodies ...
  • Description
    Mouse monoclonal [2B11AE8] to ETFA
  • Tested applicationsIP, WB, ICC/IF, In-Cell ELISA, Flow Cyt, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Human recombinant full length protein ETFA

  • Positive controlHeLa cells Human heart tissue HepG2 whole cells Human liver mitochondria Bovine heart mitochondria Rat liver mitochondria Mouse liver mitochondria

Properties

Applications

Our Abpromise guarantee covers the use of ab110316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP IP: Use at an assay dependent dilution.
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.
ICC/IF ICC/IF: Use a concentration of 4 µg/ml.
In-Cell ELISA In-Cell ELISA: Use a concentration of 4 µg/ml.
Flow Cyt Flow Cyt: Use a concentration of 1 µg/ml.
IHC-P IHC-P: Use a concentration of 5 µg/ml.

Target

  • FunctionThe electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
  • Involvement in diseaseDefects in ETFA are the cause of glutaric aciduria type 2A (GA2A) [MIM:231680]; also known as glutaricaciduria IIA. GA2A is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.
  • Sequence similaritiesBelongs to the ETF alpha-subunit/FixB family.
  • Post-translational
    modifications    The N-terminus is blocked.
  • Cellular localizationMitochondrion matrix.

    • Target information above from: UniProt accession P13804 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      Alpha ETF antibodyAlpha-ETF antibodyElectron transfer flavoprotein alpha polypeptide antibody
      Electron transfer flavoprotein alpha subunit antibodyElectron transfer flavoprotein subunit alpha mitochondrial antibodyElectron transfer flavoprotein subunit alpha, mitochondrial antibodyElectron transferring flavoprotein alpha polypeptide antibodyEMA antibodyETFA antibodyETFA_HUMAN antibodyGA2 antibodyGlutaric aciduria II antibody
    see all

Anti-ETFA antibody [2B11AE8] images

  • Immunocytochemistry/ Immunofluorescence - Anti-ETFA antibody [2B11AE8] (ab110316)
    Immunocytochemistry/ Immunofluorescence - Anti-ETFA antibody [2B11AE8] (ab110316)
    Immunocytochemistry image of stained HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab110316, 4 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix
  • Western blot - Anti-ETFA antibody [2B11AE8] (ab110316)
    Western blot - Anti-ETFA antibody [2B11AE8] (ab110316)
    All lanes : Anti-ETFA antibody [2B11AE8] (ab110316) at 1 µg/ml

    Lane 1 : Human heart tissue
    Lane 2 : HepG2 whole cells
    Lane 3 : Human liver mitochondria
    Lane 4 : Bovine heart mitochondria
    Lane 5 : Rat liver mitochondria
    Lane 6 : Mouse liver mitochondria

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 35 kDa
  • Flow Cytometry - Anti-ETFA antibody [2B11AE8] (ab110316)
    Flow Cytometry - Anti-ETFA antibody [2B11AE8] (ab110316)
    HL-60 cells were stained with 1 µg/mL ab110316 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody [2B11AE8] (ab110316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody [2B11AE8] (ab110316)

    IHC image of ETFA staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110316, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ETFA antibody [2B11AE8] (ab110316)

ab110316 has not yet been referenced specifically in any publications.

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