Anti-ETFA antibody [2B11AE8] (ab110316)

  Immunocytochemistry/ Immunofluorescence - Anti-ETFA antibody [2B11AE8] (ab110316)

Immunocytochemistry image of stained HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab110316, 4 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix

  Western blot - Anti-ETFA antibody [2B11AE8] (ab110316)

All lanes : Anti-ETFA antibody [2B11AE8] (ab110316) at 1 µg/ml

Lane 1 : Human heart tissue
Lane 2 : HepG2 whole cells
Lane 3 : Human liver mitochondria
Lane 4 : Bovine heart mitochondria
Lane 5 : Rat liver mitochondria
Lane 6 : Mouse liver mitochondria

Lysates/proteins at 10 µg per lane.


Predicted band size : 35 kDa

  Flow Cytometry - Anti-ETFA antibody [2B11AE8] (ab110316)

HL-60 cells were stained with 1 µg/mL ab110316 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody [2B11AE8] (ab110316)

IHC image of ETFA staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110316, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.