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Western blot - MADD antibody (ab2043)
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Immunocytochemistry/ Immunofluorescence - MADD antibody (ab2043)
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Product Name
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MADD antibody
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See all MADD antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MADD
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Immunogen
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Peptide corresponding to amino acids 1570 to 1588 of human MADD (1). The sequence of immunogenic peptideis identical to that of DENN and differs by one amino acidwith rat GDP/GTP exchange protein Rab3-GEP (2,5).
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Reacts with
(species key)
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Hu, Ms
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Epitope
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AAVHSSEEDLRTPPRPVSS
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: 1/250 - 1/500. Predicted molecular weight: 220 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell lysate or 3T3 whole cell lysate.
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Cellular localization
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Mitochondrion matrix
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Mitochondrial
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Relevance
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ETFA (MADD) participates in catalyzing the initial step of the mitochondrial fatty acid beta oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric,2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MADD antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MADD antibody images, references, products related to ab2043 and other tools.
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MADD antibody images:
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 Western blot - MADD antibody (ab2043)
All lanes : MADD antibody (ab2043) at 1/250 dilution
Lane 1 : Hela whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : NIH3T3 whole cell lysate
Predicted band size : 220 kDa
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Immunocytochemistry/ Immunofluorescence - MADD antibody (ab2043)
ab2043 at 10µg/ml staining MADD in human spleen tissue by ICC
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Search PubMed (MEDLINE) for references to MADD
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MADD antibody - more information
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Customer reviews (feedback) regarding MADD antibody |
Customer FAQs regarding MADD antibody |
Protocols for MADD antibody |
Price and availability of products related to MADD antibody |
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MADD antibody - related products:
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MADD antibody - other tools:
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Contact Abcam with a Technical Enquiry about MADD antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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