• Product name
  • Description
    Mouse monoclonal to MADH7
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 160-261 of Human MADH7

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 



Our Abpromise guarantee covers the use of ab55493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 46 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • Function
    Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
  • Tissue specificity
    Ubiquitous with higher expression in the lung and vascular endothelium.
  • Involvement in disease
    Colorectal cancer 3
  • Sequence similarities
    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1.
    Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation.
    Acetylation prevents ubiquitination and degradation mediated by SMURF1.
  • Cellular localization
    Nucleus. Cytoplasm. Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236).
  • Information by UniProt
  • Database links
  • Alternative names
    • CRCS3 antibody
    • FLJ16482 antibody
    • hSMAD 7 antibody
    • hSMAD7 antibody
    • MAD (mothers against decapentaplegic Drosophila) homolog 7 antibody
    • MAD antibody
    • Mad homolog 7 antibody
    • MAD homolog 8 antibody
    • MAD mothers against decapentaplegic homolog 7 antibody
    • MADH 7 antibody
    • MADH 8 antibody
    • MADH6 antibody
    • MADH8 antibody
    • Mothers Against Decapentaplegic Drosophila Homolog of 6 antibody
    • Mothers Against Decapentaplegic Drosophila Homolog of 7 antibody
    • Mothers against decapentaplegic homolog 7 antibody
    • Mothers against decapentaplegic homolog 8 antibody
    • Mothers against DPP homolog 7 antibody
    • Mothers against DPP homolog 8 antibody
    • SMAD 7 antibody
    • SMAD antibody
    • SMAD family member 7 antibody
    • SMAD, mothers against DPP homolog 7 (Drosophila) antibody
    • SMAD, mothers against DPP homolog 7 antibody
    • SMAD6 antibody
    • Smad7 antibody
    • SMAD7_HUMAN antibody
    see all


  • Overlay histogram showing A549 cells stained with ab55493 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55493, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Predicted band size : 46 kDa
    MADH7 antibody (ab55493) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • IHC image of ab55493 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab55493, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Sarenac T  et al. Single-cell analysis reveals IGF-1 potentiation of inhibition of the TGF-ß/Smad pathway of fibrosis in human keratocytes in vitro. Sci Rep 6:34373 (2016). Read more (PubMed: 27687492) »
  • Zhang LT  et al. Bone marrow-derived mesenchymal stem cells inhibit the proliferation of hepatic stellate cells by inhibiting the transforming growth factor ß pathway. Mol Med Rep 12:7227-32 (2015). Read more (PubMed: 26458849) »

See all 3 Publications for this product

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