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Western blot - MAEA antibody (ab65239)
(enlarge)
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Product Name
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MAEA antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MAEA
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Immunogen
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Synthetic peptide derived from an internal sequence of human MAEA.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab65239 detects endogenous levels of total MAEA protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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COLO205 cell extracts.
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Cellular localization
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Nucleus matrix.
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Relevance
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MAEA or Erythroblast macrophage protein (Emp), is a cell attachment protein that mediates the attachment of erythroid cells to macrophages, and is required for normal differentiation of both cell lineages. In erythroid cells MAEA is believed to be involved in nuclear extrusion however, its role in macrophage differentiation is unknown.
MAEA or Erythroblast macrophage protein (Emp) mediates the attachment of erythroblasts to macrophages within erythroblastic islands that are formed during mammalian erythropoiesis in the fetal liver and the bone marrow. Erythroblastic islands are consisted of a central macrophage surrounded by erythroblasts of varying maturity. Extensive cell:cell interactions, both erythroblast-macrophage and erythroblast:erythroblast occur within these islands.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAEA antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAEA antibody images, references, products related to ab65239 and other tools.
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MAEA antibody images:
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 Western blot - MAEA antibody (ab65239)
All lanes : MAEA antibody (ab65239) at 1/500 dilution
Lane 1 : COLO205 cell extract
Lane 2 : COLO205 cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 45 kDa
Observed band size : 45 kDa
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Search PubMed (MEDLINE) for references to MAEA
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MAEA antibody - more information
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Customer reviews (feedback) regarding MAEA antibody |
Customer FAQs regarding MAEA antibody |
Protocols for MAEA antibody |
Price and availability of products related to MAEA antibody |
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MAEA antibody - related products:
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MAEA antibody - other tools:
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Contact Abcam with a Technical Enquiry about MAEA antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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