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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Overview
Product name
Anti-MAEA antibody
See all MAEA products (2) ...
Description
Rabbit polyclonal to MAEA
Specificity
ab65239 detects endogenous levels of total MAEA protein.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from an internal sequence of human MAEA.
Positive control
COLO205 cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Erythrocytic Lineage
Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Western blot - MAEA antibody (ab65239)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
WB: 1/500 - 1/1000.Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Target
Function
Play a role in erythroblast enucleation and in the development of the mature macrophages. Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence of MAEA at cell surface of mature macrophages; This MAEA-mediated contact inhibits erythroid cells apoptosis. Participates to erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events.
Tissue specificity
Ubiquitous.
Sequence similarities
Contains 1 CTLH domain.
Contains 1 LisH domain.
Developmental stage
Localized with condensed chromatin at prophase; Detected in nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis.
Cellular localization
Nucleus matrix. Cell membrane. Cytoplasm > cytoskeleton. Localized as nuclear speckled-like pattern.
Target information above from: UniProt accessionQ7L5Y9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EMP antibody
- 1110030D19Rik antibody
- Cell proliferation inducing gene 5 protein antibody
see all
Database links
- Entrez Gene: 10296 Human
- Entrez Gene: 59003 Mouse
- Entrez Gene: 298982 Rat
- Omim: 606801 Human
- SwissProt: Q7L5Y9 Human
- SwissProt: Q4VC33 Mouse
- SwissProt: Q5RKJ1 Rat
- Unigene: 139896 Human
see all
Anti-MAEA antibody images:
Western blot - MAEA antibody (ab65239)
All lanes : Anti-MAEA antibody (ab65239) at 1/500 dilution
Lane 1 : COLO205 cell extract
Lane 2 : COLO205 cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 45 kDa
Observed band size : 45 kDa
References for Anti-MAEA antibody (ab65239)
ab65239 has not yet been referenced specifically in any publications.
Publishing research using ab65239? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"