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Products:Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Anti-MAFA antibody - ChIP Grade (ab17976)
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ab46666
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab17976? Please let us know so that we can cite the reference in this datasheet
ab17976 has been referenced in 1 publications.
- Vargas N et al. TAT-Mediated Transduction of MafA Protein In Utero Results in Enhanced Pancreatic Insulin Expression and Changes in Islet Morphology. PLoS One 6:e22364 (2011). WB; Mouse. PubMed: 21857924
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
- MAFA antibody - ChIP Grade (ab17976)
Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody.
Electrophoretic Mobility Shift Assay - MAFA antibody - ChIP Grade (ab17976)
Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069, anti-MafB Antibody or anti-c-Maf antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAFA antibody - ChIP Grade (ab17976)
ab17976 staining MAFA in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 30 minutes at 22ºC; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in blocking buffer) for 16 hours at 22ºC. A Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Western blot - Anti-MAFA antibody - ChIP Grade (ab17976)
Detection of MafA, MafB and cMaf by Western Blot. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody, anti-MafB Antibody or anti-c-Maf antibody. Each antibody was used at 0.5 ug/ml. Detection: Chemiluminescence with a 5 second exposure.
MAFA antibody - ChIP Grade for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (17976)
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