Anti-MAFA antibody - ChIP Grade (ab17976)

Thanks for you good service. I am very interested in the MafA marker or PDX-1 markers that works in mouse/rat pancreas (paraffin sections). Instead of a refund I would be willing to test a new vial (lot number) of the MafA - or test some of your PDX-1 markers. I am very interested in beta cell specific nuclei markers.
Would you be able to help me on this issue - in return I will procide you with an online officiel abreview based on the results.

ANSWER:

Thank you for your reply and the nice feedback.
I have checked our stock, and it seems we only have the same lot number available you had previously. I can assure you that you have our Abpromise on every replacement as well if this one would fail as well.
On the otherside, there is no problem of sending you a PDX-1 marker or a different MafA antibody as a replacement.
Did both orders fail to work or only one of them? If it is one, which order was it?
Looking forward to your reply.

1. What kind of species was used? The ab has been tested in mice (NOD mice and db/db mice) and in rat
2. What was the antibody incubation time and temperature? Ab incubation time was 1 hr at room temperature.
3. When did you order the antibody? We have ordered two batches of MafA - as we ended up with a very strong dilution (1:50). The order was given Marts 12, 2012.

ANSWER:

Thank you for taking time to answer my questions.
Having reviewed the additional protocol details, I believe this product should have given satisfactory results. It appears that you may have received two faulty vials, which is regrettable as this only the second complaint we received since we published this antibody in 2005: this antibody is usually very reliable.
I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation for both vials.
Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

We have been testing your MafA antibody (17976) in several testing conditions on paraffin embedded sections: 1:50-1:400, using standard ABC visualizations systems as well as MACH2 or Envision amplification. We get no labelling except when using MACH2 amplication and low dilution MafA ab (1:50) - this staining appears however to be non-specific (see attached image revealing staining of the whole cytosol and not in nuclei only). I would be interested in your opinion on this issue - otherwise I would like to have a refund or try another beta cell specific marker (e.g. one of your PDX-1 abs).
"We provide expert scientific support for all of our products to enable you to achieve the result you should expect (we have over 40 PhDs in-house to answer your queries). We guarantee that all products will work as described on our datasheets, or we will give you a replacement or refund".

ANSWER:

Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.