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Anti-MAFA antibody - ChIP Grade (ab17976)

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Overview

Product name

Anti-MAFA antibody - ChIP Grade
See all MAFA products (5) ...

Description

Rabbit polyclonal to MAFA - ChIP Grade

Specificity

Antibody has not been used successfully for the human ortholog.

Tested applications

WB, ChIP, EMSA, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Mouse

Immunogen

Synthetic peptide, represented a domain of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (Genbank ID 389692) located near the C-terminus.

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • - MAFA antibody - ChIP Grade (ab17976)- MAFA antibody - ChIP Grade (ab17976) image (enlarge)

  • Electrophoretic Mobility Shift Assay - MAFA antibody - ChIP Grade (ab17976)Electrophoretic Mobility Shift Assay - MAFA antibody - ChIP Grade (ab17976) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAFA antibody - ChIP Grade (ab17976)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAFA antibody - ChIP Grade (ab17976) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab17976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.

Sequence similarities

Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.

Post-translational
modifications

Ubiquitinated, leading to its degradation by the proteasome.
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.

Cellular localization

Nucleus. Detected in nuclei of pancreas islet beta cells.

Target information above from: UniProt accessionQ8NHW3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • HMafA antibody
  • Mafa antibody
  • MAFA_HUMAN antibody
  • Pancreatic beta cell specific transcriptional activator antibody
  • Pancreatic beta-cell-specific transcriptional activator antibody
  • RIPE3b1 antibody
  • Transcription factor MafA antibody
  • Transcription factor RIPE3b1 antibody
  • v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian) antibody
  • v maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
  • V-maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody
see all

Anti-MAFA antibody - ChIP Grade images:

  - MAFA antibody - ChIP Grade (ab17976)

- MAFA antibody - ChIP Grade (ab17976)

Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody.

  Electrophoretic Mobility Shift Assay - MAFA antibody - ChIP Grade (ab17976)

Electrophoretic Mobility Shift Assay - MAFA antibody - ChIP Grade (ab17976)

Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069, anti-MafB Antibody or anti-c-Maf antibody.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAFA antibody - ChIP Grade (ab17976)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAFA antibody - ChIP Grade (ab17976)

ab17976 staining MAFA in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in blocking buffer) for 16 hours at 22°C. A Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

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  Western blot - Anti-MAFA antibody - ChIP Grade (ab17976)

Western blot - Anti-MAFA antibody - ChIP Grade (ab17976)

Detection of MafA, MafB and cMaf by Western Blot. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody, anti-MafB Antibody or anti-c-Maf antibody. Each antibody was used at 0.5 ug/ml. Detection: Chemiluminescence with a 5 second exposure.

References for Anti-MAFA antibody - ChIP Grade (ab17976)

This product has been referenced in:

  • Vargas Net al. TAT-Mediated Transduction of MafA Protein In Utero Results in Enhanced Pancreatic Insulin Expression and Changes in Islet Morphology. PLoS One 6:e22364 (2011). WB; Mouse.Read more (PubMed: 21857924) »

See 1 publication for this product

Publishing research using ab17976? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"