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- MAFA antibody - ChIP Grade (ab17976)
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Electrophoretic Mobility Shift Assay - MAFA antibody - ChIP Grade (ab17976)
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- MAFA antibody - ChIP Grade (ab17976)
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Product Name
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MAFA antibody - ChIP Grade
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See all MAFA antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MAFA - ChIP Grade
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Immunogen
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Synthetic peptide, represented a domain of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (Gengbank ID 389692) located near the C-terminus.
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Reacts with
(species key)
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Ms
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Specificity
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Antibody has not been used successfully for the human ortholog.
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Tested applications
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ChIP, EMSA, IHC-Fr, WB
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Abreviews
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Application notes
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Recommended dilutions
ChIP: Use 5-15µg for 108 cells.
EMSA: Use at a concentration of 0.05 - 0.25 mg/ml.
IHC-Fr: 1/1000 - 1/3000.
WB: 1/1000 - 1/10000. Predicted molecular weight: 37.5 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
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Relevance
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Insulin gene expression is regulated by several islet-enriched transcription factors. However, MAFA is the only beta cell-specific activator. MAFA selectively induces endogenous insulin transcription in non-beta cells. MAFA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MAFA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1 % Sodium Azide
Constituents: Tris citrate/phosphate. pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAFA antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAFA antibody - ChIP Grade images, references, products related to ab17976 and other tools.
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MAFA antibody - ChIP Grade images:
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 - MAFA antibody - ChIP Grade (ab17976)
Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody.
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Electrophoretic Mobility Shift Assay - MAFA antibody - ChIP Grade (ab17976)
Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069, anti-MafB Antibody or anti-c-Maf antibody.
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- MAFA antibody - ChIP Grade (ab17976)
Predicted band size : 21 kDa
Detection of MafA, MafB and cMaf by Western Blot. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody, anti-MafB Antibody or anti-c-Maf antibody. Each antibody was used at 0.5 ug/ml. Detection: Chemiluminescence with a 5 second exposure.
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References for MAFA antibody - ChIP Grade (ab17976)
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This product has been used in: (two most recent references)
Matsuoka TA et al. The MafA transcription factor appears to be responsible for tissue-specific expression of insulin. Proc Natl Acad Sci U S A 101:2930-3 (2004). PubMed: 14973194
Matsuoka TA et al. Members of the large Maf transcription family regulate insulin gene transcription in islet beta cells. Mol Cell Biol 23:6049-62 (2003). PubMed: 12917329
If you publish research using ab17976 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MAFA
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MAFA antibody - ChIP Grade - more information
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