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Products:Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Overview
Product name
Anti-MAFA antibody - ChIP Grade
See all MAFA products (5) ...
Description
Rabbit polyclonal to MAFA - ChIP Grade
Specificity
Antibody has not been used successfully for the human ortholog.
Tested applications
WB, ChIP, EMSA, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Synthetic peptide, represented a domain of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (Genbank ID 389692) located near the C-terminus.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000.
ChIP: Use 5-15µg for 108 cells.
EMSA: Use a concentration of 0.05 - 0.25 mg/ml.
IHC-P: Use at an assay dependent dilution.
IHC-Fr
IHC-Fr: 1/1000 - 1/3000.IHC-Fr: 1/1000 - 1/3000.
Target
Function
Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
Sequence similarities
Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.
Post-translational
modifications
Ubiquitinated, leading to its degradation by the proteasome.
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
Cellular localization
Nucleus. Detected in nuclei of pancreas islet beta cells.
Target information above from: UniProt accessionQ8NHW3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HMafA antibody
- Mafa antibody
- MAFA_HUMAN antibody
see all
Database links
- Entrez Gene: 378435 Mouse
- SwissProt: Q8CF90 Mouse
- Unigene: 309589 Mouse
Anti-MAFA antibody - ChIP Grade images:
- MAFA antibody - ChIP Grade (ab17976)
Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody.
Electrophoretic Mobility Shift Assay - MAFA antibody - ChIP Grade (ab17976)
Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069, anti-MafB Antibody or anti-c-Maf antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAFA antibody - ChIP Grade (ab17976)
ab17976 staining MAFA in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in blocking buffer) for 16 hours at 22°C. A Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Western blot - Anti-MAFA antibody - ChIP Grade (ab17976)
Detection of MafA, MafB and cMaf by Western Blot. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody, anti-MafB Antibody or anti-c-Maf antibody. Each antibody was used at 0.5 ug/ml. Detection: Chemiluminescence with a 5 second exposure.
References for Anti-MAFA antibody - ChIP Grade (ab17976)
This product has been referenced in:
- Vargas Net al. TAT-Mediated Transduction of MafA Protein In Utero Results in Enhanced Pancreatic Insulin Expression and Changes in Islet Morphology. PLoS One 6:e22364 (2011). WB; Mouse.Read more (PubMed: 21857924) »
See 1 publication for this product
Publishing research using ab17976? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"