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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab26405? Please let us know so that we can cite the reference in this datasheet
ab26405 has been referenced in 2 publications.
- Zhang T et al. Functional role of an islet transcription factor, INSM1/IA-1, on pancreatic acinar cell trans-differentiation. J Cell Physiol : (2011). PubMed: 21830214
- Kondo T et al. p38 MAPK is a major regulator of MafA protein stability under oxidative stress. Mol Endocrinol 23:1281-90 (2009). WB. PubMed: 19407223
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAFA antibody (ab26405)
The image shows human pancreas tissue in which some cells exhibit nuclear staining. The antigen retrieval method used was Tris EDTA pH9.
Western blot - MAFA antibody (ab26405)
All lanes : Anti-MAFA antibody (ab26405) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate
Lane 2 : Kidney (Human) Tissue Lysate
Lane 3 : Liver (Human) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data), however publications have shown the human protein to be detected at 47 kDa (PMID 12011435 ). Our data shows ab26405 to detect a band at 36 kDa in human brain, kidney and liver which is similar to that seen in other publications (PMID:14680841, PMID:18159220 (expression profile) ). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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