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Anti-MAFA antibody (ab26405)

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab26405 for help.

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1 question for ab26405

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Question 1

Thursday 15-December-2011

Dear technical support member,

I am using your anti-MAFA antibody (ab26405) and have a question

related to the unmasking procedure I should use. According to the data

sheet you suggest to use Tris-EDTA buffer (pH9), but there is no

information about the steps to follow in your website; would you

please send me a detailed protocol.

ANSWER:

  

Thank you for contacting us.

The IHC image on the datasheet for ab26405 was provided by a collaborator and we unfortunately do not have their exact protocol. However, it is safe to assume that the protocol is standard and that the unmasking procedure was similar to the procedure described in the PDF file I have attached, in section D: Antigen Retrieval. This general protocol can also be found on our website at this link:

http://www.abcam.com/ps/pdf/protocols/ihc_p.pdf

Section D contains a recipe for Tris-EDTA pH 9.0 and recommendations for heating. Briefly, if using a microwave or steamer or water bath, you will want to maintain the slides in the buffer at 95-100C for 10 - 20 minutes, followed by cooling for another 20 minutes. The optimal amount of time for your tissue sections will need to be determined but in general, 20 minutes at 95-100C is usually sufficient.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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