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Products:Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Overview
Product name
Anti-MAFA antibody
See all MAFA products (5) ...
Description
Rabbit polyclonal to MAFA
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human MAFA.
(Peptide available as ab28523.)
Positive control
ab26405 gave a positive signal in the following human tissue lysates: Brain Kidney Liver
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: Use at a concentration of 0.8 - 4 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data), however publications have shown the human protein to be detected at 47 kDa according to reference PMID 12011435, http://www.pnas.org/cgi/content/full/99/10/6737. Our data shows ab26405 to detect a band at 36 kDa in human brain, kidney and liver which is similar to that seen in other publications (PMID:14680841, PMID:18159220 (expression profile) ).
The mouse protein has also been observed at 47 kDa according to reference PMID 12368292.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
Sequence similarities
Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.
Post-translational
modifications
Ubiquitinated, leading to its degradation by the proteasome.
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
Cellular localization
Nucleus. Detected in nuclei of pancreas islet beta cells.
Target information above from: UniProt accessionQ8NHW3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HMafA antibody
- Mafa antibody
- MAFA_HUMAN antibody
see all
Database links
- Entrez Gene: 389692 Human
- Omim: 610303 Human
- SwissProt: Q8NHW3 Human
- Unigene: 521914 Human
Anti-MAFA antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAFA antibody (ab26405)
The image shows human pancreas tissue in which some cells exhibit nuclear staining. The antigen retrieval method used was Tris EDTA pH9.
Western blot - MAFA antibody (ab26405)
All lanes : Anti-MAFA antibody (ab26405) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate
Lane 2 : Kidney (Human) Tissue Lysate
Lane 3 : Liver (Human) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data), however publications have shown the human protein to be detected at 47 kDa (PMID 12011435 ). Our data shows ab26405 to detect a band at 36 kDa in human brain, kidney and liver which is similar to that seen in other publications (PMID:14680841, PMID:18159220 (expression profile) ). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
References for Anti-MAFA antibody (ab26405)
This product has been referenced in:
- Zhang Tet al. Functional role of an islet transcription factor, INSM1/IA-1, on pancreatic acinar cell trans-differentiation. J Cell Physiol : (2011).Read more (PubMed: 21830214) »
- Kondo Tet al. p38 MAPK is a major regulator of MafA protein stability under oxidative stress. Mol Endocrinol 23:1281-90 (2009). WB.Read more (PubMed: 19407223) »
See all 2 publications for this product
Publishing research using ab26405? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"