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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAFA antibody (ab26405)
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Western blot - MAFA antibody (ab26405)
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Product Name
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MAFA antibody
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See all MAFA antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MAFA
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human MAFA.
(Peptide available as ab28523.)
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Reacts with
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Hu
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Does not react with
(species key)
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Ms
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 0.8 - 4 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data), however publications have shown the human protein to be detected at 47 kDa according to reference PMID 12011435, http://www.pnas.org/cgi/content/full/99/10/6737. Our data shows ab26405 to detect a band at 36 kDa in human brain, kidney and liver which is similar to that seen in other publications (PMID:14680841, PMID:18159220 (expression profile) ).
The mouse protein has also been observed at 47 kDa according to reference PMID 12368292.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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ab26405 gave a positive signal in the following human tissue lysates:
Brain
Kidney
Liver
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
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Relevance
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Insulin gene expression is regulated by several islet-enriched transcription factors. However, MAFA is the only beta cell-specific activator. MAFA selectively induces endogenous insulin transcription in non-beta cells. MAFA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MAFA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.30 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAFA antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAFA antibody images, references, products related to ab26405 and other tools.
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MAFA antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAFA antibody (ab26405)
The image shows human pancreas tissue in which some cells exhibit nuclear staining. The antigen retrieval method used was Tris EDTA pH9.
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Western blot - MAFA antibody (ab26405)
All lanes : MAFA antibody (ab26405) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate
Lane 2 : Kidney (Human) Tissue Lysate
Lane 3 : Liver (Human) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data), however publications have shown the human protein to be detected at 47 kDa (PMID 12011435 ). Our data shows ab26405 to detect a band at 36 kDa in human brain, kidney and liver which is similar to that seen in other publications (PMID:14680841, PMID:18159220 (expression profile) ). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Search PubMed (MEDLINE) for references to MAFA
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MAFA antibody - more information
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Customer reviews (feedback) regarding MAFA antibody |
Customer FAQs regarding MAFA antibody |
Protocols for MAFA antibody |
Price and availability of products related to MAFA antibody |
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MAFA antibody - related products:
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MAFA antibody - other tools:
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Contact Abcam with a Technical Enquiry about MAFA antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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