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Products:Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Overview
Product name
Anti-MAFA antibody
See all MAFA products (5) ...
Description
Mouse monoclonal to MAFA
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: LEERFSDDQL VSMSVRELNR QLRGFSKEEV IRLKQKRRTL KNRGYAQSCR FKRVQQRHIL ESEKCQLQSQ VEQLKLEVGR LAKERDL, corresponding to amino acids 222-309 of Human MAFA
LEERFSDDQL VSMSVRELNR QLRGFSKEEV IRLKQKRRTL KNRGYAQSCR FKRVQQRHIL ESEKCQLQSQ VEQLKLEVGR LAKERDL
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
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Western blot - MAFA antibody (ab57807)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab57807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
Sequence similarities
Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.
Post-translational
modifications
Ubiquitinated, leading to its degradation by the proteasome.
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
Cellular localization
Nucleus. Detected in nuclei of pancreas islet beta cells.
Target information above from: UniProt accessionQ8NHW3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HMafA antibody
- Mafa antibody
- MAFA_HUMAN antibody
see all
Database links
- Entrez Gene: 389692 Human
- Omim: 610303 Human
- SwissProt: Q8NHW3 Human
- Unigene: 521914 Human
Anti-MAFA antibody images:
Western blot - MAFA antibody (ab57807)
Western blot against tagged recombinant protein immunogen using ab57807 MAFA antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
References for Anti-MAFA antibody (ab57807)
ab57807 has not yet been referenced specifically in any publications.
Publishing research using ab57807? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"