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Western blot - MAFA antibody (ab57807)
(enlarge)
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Product Name
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MAFA antibody
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See all MAFA antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to MAFA
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Immunogen
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Recombinant fragment: LEERFSDDQL VSMSVRELNR QLRGFSKEEV IRLKQKRRTL KNRGYAQSCR FKRVQQRHIL ESEKCQLQSQ VEQLKLEVGR LAKERDL, corresponding to amino acids 222-309 of Human MAFA
BLAST 'LEERFSDDQL VSMSVRELNR QLRGFSKEEV IRLKQKRRTL KNRGYAQSCR FKRVQQRHIL ESEKCQLQSQ VEQLKLEVGR ' with  or 
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
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Relevance
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Insulin gene expression is regulated by several islet-enriched transcription factors. However, MAFA is the only beta cell-specific activator. MAFA selectively induces endogenous insulin transcription in non-beta cells. MAFA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MAFA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAFA antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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MAFA antibody images:
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 Western blot - MAFA antibody (ab57807)
Western blot against tagged recombinant protein immunogen using ab57807 MAFA antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
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Search PubMed (MEDLINE) for references to MAFA
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MAFA antibody - more information
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Customer reviews (feedback) regarding MAFA antibody |
Customer FAQs regarding MAFA antibody |
Protocols for MAFA antibody |
Price and availability of products related to MAFA antibody |
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MAFA antibody - other tools:
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Contact Abcam with a Technical Enquiry about MAFA antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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