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All lanes : Anti-MAFA antibody (ab86020) at 1/1000 dilution
Lane 1 : Uninduced (negative control) culture of E.coli
Lane 2 : Induced culture of E.coli
Secondary
anti-Rabbit IgG HRP conjugated at 1/1000 dilution
Predicted band size : 37 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
Predicted MW of recombinant MAFA protein: 51kDa (37kDa + another 14kDa for the tag).
Gel concentration 10%
0
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