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Products:Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Overview
Product name
Anti-MAFA antibody
See all MAFA products (5) ...
Description
Rabbit polyclonal to MAFA
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
3 synthetic peptides (Human) conjugated to KLH
Positive control
Recombinant protein produced in an induced culture of E.coli.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
-
Western blot - MAFA antibody (ab86020)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 37 kDa.
Target
Function
Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
Sequence similarities
Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.
Post-translational
modifications
Ubiquitinated, leading to its degradation by the proteasome.
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
Cellular localization
Nucleus. Detected in nuclei of pancreas islet beta cells.
Target information above from: UniProt accessionQ8NHW3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HMafA antibody
- Mafa antibody
- MAFA_HUMAN antibody
see all
Database links
- Entrez Gene: 389692 Human
- Entrez Gene: 378435 Mouse
- Omim: 610303 Human
- SwissProt: Q8NHW3 Human
- SwissProt: Q8CF90 Mouse
- Unigene: 521914 Human
- Unigene: 309589 Mouse
Anti-MAFA antibody images:
Western blot - MAFA antibody (ab86020)
All lanes : Anti-MAFA antibody (ab86020) at 1/1000 dilution
Lane 1 : Uninduced (negative control) culture of E.coli
Lane 2 : Induced culture of E.coli
Secondary
anti-Rabbit IgG HRP conjugated at 1/1000 dilution
Predicted band size : 37 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
Predicted MW of recombinant MAFA protein: 51kDa (37kDa + another 14kDa for the tag).
Gel concentration 10%
References for Anti-MAFA antibody (ab86020)
ab86020 has not yet been referenced specifically in any publications.
Publishing research using ab86020? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"