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Western blot - MAFA antibody (ab86020)
(enlarge)
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Product Name
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MAFA antibody
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See all MAFA antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MAFA
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Immunogen
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3 synthetic peptides (Human) conjugated to KLH
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 37 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Recombinant protein produced in an induced culture of E.coli.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
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Relevance
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Insulin gene expression is regulated by several islet-enriched transcription factors. However, MAFA is the only beta cell-specific activator. MAFA selectively induces endogenous insulin transcription in non-beta cells. MAFA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MAFA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAFA antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAFA antibody images, references, products related to ab86020 and other tools.
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MAFA antibody images:
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 Western blot - MAFA antibody (ab86020)
All lanes : MAFA antibody (ab86020) at 1/1000 dilution
Lane 1 : Uninduced (negative control) culture of E.coli
Lane 2 : Induced culture of E.coli
Secondary
anti-Rabbit IgG HRP conjugated at 1/1000 dilution
Predicted band size : 37 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
Predicted MW of recombinant MAFA protein: 51kDa (37kDa + another 14kDa for the tag).
Gel concentration 10%
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Search PubMed (MEDLINE) for references to MAFA
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MAFA antibody - more information
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Customer reviews (feedback) regarding MAFA antibody |
Customer FAQs regarding MAFA antibody |
Protocols for MAFA antibody |
Price and availability of products related to MAFA antibody |
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MAFA antibody - related products:
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MAFA antibody - other tools:
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Contact Abcam with a Technical Enquiry about MAFA antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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