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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab89780? Please let us know so that we can cite the reference in this datasheet
ab89780 has been referenced in 1 publications.
- Jin F et al. N-WASp is required for Schwann cell cytoskeletal dynamics, normal myelin gene expression and peripheral nerve myelination. Development 138:1329-37 (2011). WB; Mouse. PubMed: 21385763
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG antibody (ab89780)
IHC-P image of MAG staining on cow peripheral nerve sections using ab89780 (1:4000). The sections were fixed in Formaldehyde and subjected to heat mediated antigen retrieval using citric acid (pH 6). The sections were blocked using 1% BSA for 10 mins at 21ºC.
This image was taken from an abreview submitted by Carl Hobbs, King`s College London.
Western blot - MAG antibody (ab89780)
Anti-MAG antibody (ab89780) at 5 µg/ml + Partial tagged recombinant Human MAG protein at 0.2 µg
Predicted band size : 63 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 36 kDa.
Western blot - MAG antibody (ab89780)
Anti-MAG antibody (ab89780) at 5 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size : 63 kDa
Observed band size : 53 kDa (why is the actual band size different from the predicted?)
Flow Cytometry-MAG antibody(ab89780)
Overlay histogram showing SH-SY5Y cells stained with ab89780 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89780, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
MAG antibody for Western blot in Mouse (89780)
MAG antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (89780)
MAG antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Rat (89780)
MAG antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Cow (89780)
MAG antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (89780)
MAG antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Monkey (89780)
Anti-MAG antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Cat (89780)
MAG antibody for Western blot in Rabbit (89780)
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