Anti-MAG antibody (ab89780)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG antibody (ab89780)

IHC-P image of MAG staining on cow peripheral nerve sections using ab89780 (1:4000). The sections were fixed in Formaldehyde and subjected to heat mediated antigen retrieval using citric acid (pH 6). The sections were blocked using 1% BSA for 10 mins at 21°C.

This image was taken from an abreview submitted by Carl Hobbs, King`s College London.

Western blot - MAG antibody (ab89780)

Anti-MAG antibody (ab89780) at 5 µg/ml + Partial tagged recombinant Human MAG protein at 0.2 µg

Predicted band size : 63 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)


Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 36 kDa.

Western blot - MAG antibody (ab89780)

Anti-MAG antibody (ab89780) at 5 µg/ml + Jurkat cell lysate at 50 µg

Predicted band size : 63 kDa
Observed band size : 53 kDa (why is the actual band size different from the predicted?)

Flow Cytometry-MAG antibody(ab89780)

Overlay histogram showing SH-SY5Y cells stained with ab89780 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89780, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.