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Immunocytochemistry/ Immunofluorescence - MAG1 antibody (ab64979)
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Western blot - MAG1 antibody (ab64979)
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Product Name
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MAG1 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MAG1
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Immunogen
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Synthesized peptide derived from C-terminal of human PLCH.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Detects endogenous levels of total PLCH protein.
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Tested applications
(see key)
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Several other bands are also visible; we have no explanation for their presence.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from Jurkat, HeLa, and COLO205 cells. HepG2 cells.
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Cellular localization
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Endoplasmic reticulum membrane; Multi-pass membrane protein.
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Research areas
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Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
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Relevance
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MAG1 is the endoplasmic reticulum form of acyl-CoA:glycerol-3-phosphate acyltransferase - which catalyzes the initial step of de novo triacylglycerol synthesis by converting glycerol-3-phosphate to lysophosphatidic acid. Overexpression of MAG1 activates the mTOR pathway.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAG1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAG1 antibody images, references, products related to ab64979 and other tools.
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MAG1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - MAG1 antibody (ab64979)
This image shows HepG2 cells stained with ab64979 at 1/500 - 1/1000 dilution; with and without the immunising peptide.
This image shows HepG2 cells stained with ab64979 at 1/500 -1/1000 dilution; with and without the immunising peptide.
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Western blot - MAG1 antibody (ab64979)
All lanes : MAG1 antibody (ab64979) at 1/500 dilution
Lane 1 : extracts from Jurkat cells (5-30µg total protein)
Lane 2 : extracts from HeLa cells (5-30µg total protein)
Lane 3 : extracts from COLO205 cells (5-30µg total protein)
Lane 4 : extracts from COLO205 cells (5-30µg total protein) and 5-10µg of the immunising peptide
Predicted band size : 49 kDa
Observed band size : 49 kDa
Additional bands at : 117 kDa,22 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to MAG1
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MAG1 antibody - more information
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Customer reviews (feedback) regarding MAG1 antibody |
Customer FAQs regarding MAG1 antibody |
Protocols for MAG1 antibody |
Price and availability of products related to MAG1 antibody |
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MAG1 antibody - related products:
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MAG1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MAG1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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