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Anti-MAG1 antibody (ab64979)

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Overview

Product name

Anti-MAG1 antibody
See all MAG1 products (3) ...

Description

Rabbit polyclonal to MAG1

Specificity

Detects endogenous levels of total PLCH protein.

Tested applications

WB, ELISA, ICC/IFmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthesized peptide derived from C-terminal of human PLCH.

Positive control

Extracts from Jurkat, HeLa, and COLO205 cells. HepG2 cells.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other

  • Immunocytochemistry/ Immunofluorescence - MAG1 antibody (ab64979)Immunocytochemistry/ Immunofluorescence - MAG1 antibody (ab64979) image (enlarge)

  • Western blot - MAG1 antibody (ab64979)Western blot - MAG1 antibody (ab64979) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab64979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: 1/5000.
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Several other bands are also visible; we have no explanation for their presence.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Overexpression activates the mTOR pathway.

Tissue specificity

Widely expressed. Expressed in liver, kidney, testis, brain, heart, skeletal muscle, thyroid, prostate, thymus and placenta. Also expressed lung and adipose tissue.

Pathway

Glycerolipid metabolism; triacylglycerol biosynthesis.
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.

Sequence similarities

Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Domain

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.

Cellular localization

Endoplasmic reticulum membrane.

Target information above from: UniProt accessionQ53EU6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • PLCH antibody
  • 1 AGP acyltransferase 9 antibody
  • 1 acylglycerol 3 phosphate O acyltransferase 8 antibody
  • 1 acylglycerol 3 phosphate O acyltransferase 9 antibody
  • 1 AGPAT 9 antibody
  • 1-acylglycerol-3-phosphate O-acyltransferase 9 antibody
  • 1-AGP acyltransferase 9 antibody
  • 1-AGPAT 9 antibody
  • Acyl CoA:glycerol 3 phosphate acyltransferase 3 antibody
  • Acyl-CoA:glycerol-3-phosphate acyltransferase 3 antibody
  • AGPAT8 antibody
  • AGPAT9 antibody
  • Glycerol-3-phosphate acyltransferase 3 antibody
  • GPAT-3 antibody
  • GPAT3 antibody
  • GPAT3_HUMAN antibody
  • hGPAT3 antibody
  • LPAAT theta antibody
  • LPAAT-theta antibody
  • Lung cancer metastasis associated protein antibody
  • Lung cancer metastasis-associated protein 1 antibody
  • Lysophosphatidic acid acyltransferase theta antibody
  • MAG 1 antibody
  • MAG-1 antibody
  • MGC11324 antibody
see all

Database links

see all

Anti-MAG1 antibody images:

  Immunocytochemistry/ Immunofluorescence - MAG1 antibody (ab64979)

Immunocytochemistry/ Immunofluorescence - MAG1 antibody (ab64979)

This image shows HepG2 cells stained with ab64979 at 1/500 -1/1000 dilution; with and without the immunising peptide.

  Western blot - MAG1 antibody (ab64979)

Western blot - MAG1 antibody (ab64979)

All lanes : Anti-MAG1 antibody (ab64979) at 1/500 dilution

Lane 1 : extracts from Jurkat cells (5-30µg total protein)
Lane 2 : extracts from HeLa cells (5-30µg total protein)
Lane 3 : extracts from COLO205 cells (5-30µg total protein)
Lane 4 : extracts from COLO205 cells (5-30µg total protein) and 5-10µg of the immunising peptide


Predicted band size : 49 kDa
Observed band size : 49 kDa
Additional bands at : 117 kDa,22 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MAG1 antibody (ab64979)

ab64979 has not yet been referenced specifically in any publications.

Publishing research using ab64979? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"