|
|
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGE 1 antibody (ab21472)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - MAGE 1 antibody (ab21472)
(enlarge)
|
|
|
|
|
Product Name
|
MAGE 1 antibody
|
| |
See all MAGE 1 antibodies (9)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to MAGE 1
|
|
Immunogen
|
Synthetic peptide: QEKYLEYRQVPDSDP, corresponding to amino acids 245-259 of Human MAGE 1.
BLAST 'QEKYLEYRQVPDSDP' with  or 
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ELISA, ICC/IF, IHC-P, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: Use at a concentration of 0.1 - 1 µg/ml.
ICC: 1/20.
ICC/IF: Used at a dilution of 1/25 (see Abreview).
IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use 3µg for 107 cells.
WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 34 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Human melanoma
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
|
|
Relevance
|
The MAGE 1 gene is a member of the melanoma antigen encoding gene family.
These genes encode for HLA-restricted tumor associated rejection antigens recognized by cytotoxic T lymphocytes. Some of these target antigens may be potentially useful for cancer specific immunotherapy. The expression of MAGE genes has been reported not only in melanoma but also in various other malignant tumors such as hepatocellular carcinoma and germ cell tumors.
|
|
|
MAGE 1 antibody (ab21472) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.200 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAGE 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for MAGE 1 antibody images, references, products related to ab21472 and other tools.
|
| |
MAGE 1 antibody images:
|
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGE 1 antibody (ab21472)
Immunohistochemistry of formalin-fixed human melanoma stained for MAGE 1.
|
| |
Immunocytochemistry/ Immunofluorescence - MAGE 1 antibody (ab21472)
ab21472 staining the human breast cancer cell line, MCF-7, by ICC/IF. The cell line was stimulated with 5-aza-CdR for 72 hours. The treated cells, or a mock control were then stimulated by trichostatin A for 24 hours. ab21472 was used at a dilution of 1/25 and an Alexa Fluor ® 546 conjugated goat anti-rabbit IgG was used as the secondary. This image is courtesy of an Abreview submitted by Dr H Schwarzenbach
See Abreview
|
| |
References for MAGE 1 antibody (ab21472)
|
|
This product has been used in:
Wischnewski F et al. Promoter demethylation and histone acetylation mediate gene expression of MAGE-A1, -A2, -A3, and -A12 in human cancer cells. Mol Cancer Res 4:339-49 (2006). PubMed: 16687489
If you publish research using ab21472 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to MAGE 1
|
| |
MAGE 1 antibody - more information
|
Customer reviews (feedback) regarding MAGE 1 antibody |
Customer FAQs regarding MAGE 1 antibody |
Protocols for MAGE 1 antibody |
Price and availability of products related to MAGE 1 antibody |
| |
MAGE 1 antibody - related products:
|
|
|
| |
MAGE 1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about MAGE 1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
