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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-MAGE 1 antibody
See all MAGE 1 products (9) ...
Description
Rabbit polyclonal to MAGE 1
Tested applications
ELISA, WB, IP, IHC-P, ICC, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: QEKYLEYRQVPDSDP, corresponding to amino acids 245-259 of Human MAGE 1.
QEKYLEYRQV PDSDP
Positive control
Human melanoma
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
IP
- 1 Image
IHC-P
ICC
Application notes
ELISA: Use at a concentration of 0.1 - 1 µg/ml.
ICC: 1/20.
ICC/IF: Used at a dilution of 1/25 (see Abreview).
IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use 3µg for 107 cells.
WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 34 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
The MAGE 1 gene is a member of the melanoma antigen encoding gene family. These genes encode for HLA-restricted tumor associated rejection antigens recognized by cytotoxic T lymphocytes. Some of these target antigens may be potentially useful for cancer specific immunotherapy. The expression of MAGE genes has been reported not only in melanoma but also in various other malignant tumors such as hepatocellular carcinoma and germ cell tumors.
Cellular localization
Cytoplasmic
Alternative names
- Antigen MZ2 E antibody
- MAGE 1 antigen antibody
- MAGE1 antibody
see all
Database links
- Entrez Gene: 4100 Human
- Omim: 300016 Human
- SwissProt: P43355 Human
Anti-MAGE 1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGE 1 antibody (ab21472)
Immunohistochemistry of formalin-fixed human melanoma stained for MAGE 1.
Immunocytochemistry/ Immunofluorescence - MAGE 1 antibody (ab21472)
ab21472 staining the human breast cancer cell line, MCF-7, by ICC/IF. The cell line was stimulated with 5-aza-CdR for 72 hours. The treated cells, or a mock control were then stimulated by trichostatin A for 24 hours. ab21472 was used at a dilution of 1/25 and an Alexa Fluor ® 546 conjugated goat anti-rabbit IgG was used as the secondary.
This image is courtesy of an Abreview submitted by Dr H Schwarzenbach
References for Anti-MAGE 1 antibody (ab21472)
This product has been referenced in:
- Wischnewski Fet al. Promoter demethylation and histone acetylation mediate gene expression of MAGE-A1, -A2, -A3, and -A12 in human cancer cells. Mol Cancer Res 4:339-49 (2006).Read more (PubMed: 16687489) »
See 1 publication for this product
Publishing research using ab21472? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"