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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-MAGE 1 antibody
See all MAGE 1 products (9) ...
Description
Rabbit polyclonal to MAGE 1
Specificity
This antibody is specific for MAGE 1 (N term).
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A KLH conjugated synthetic peptide (10-30 aa in length) in the region of aa 200~250 of human MAGE 1.
Positive control
Breast carcinoma tissue and Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500.Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
IHC-P: 1/50 - 1/100.
Target
Relevance
The MAGE 1 gene is a member of the melanoma antigen encoding gene family. These genes encode for HLA-restricted tumor associated rejection antigens recognized by cytotoxic T lymphocytes. Some of these target antigens may be potentially useful for cancer specific immunotherapy. The expression of MAGE genes has been reported not only in melanoma but also in various other malignant tumors such as hepatocellular carcinoma and germ cell tumors.
Cellular localization
Cytoplasmic
Alternative names
- MAGE1 antibody
- Antigen MZ2 E antibody
- MAGE 1 antigen antibody
see all
Database links
- Entrez Gene: 4100 Human
- Omim: 300016 Human
- SwissProt: P43355 Human
Anti-MAGE 1 antibody images:
Western blot - MAGE 1 antibody (ab38486)
Anti-MAGE 1 antibody (ab38486) at 1/100 dilution + Jurkat cell lysate
Predicted band size : 24 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Additional bands at : 20 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGE 1 antibody (ab38486)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38486 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
References for Anti-MAGE 1 antibody (ab38486)
ab38486 has not yet been referenced specifically in any publications.
Publishing research using ab38486? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"