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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-MAGE 1 antibody
See all MAGE 1 products (9) ...
Description
Rabbit polyclonal to MAGE 1
Specificity
ab53131 detects endogenous levels of total MAGE 1 protein.
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
A synthetic derived from human MAGE 1.
Positive control
WB: HepG2 cell extracts IHC-P: Human breast carcinoma tissue
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab53131 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/300 - 1/1000.Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Relevance
The MAGE 1 gene is a member of the melanoma antigen encoding gene family. These genes encode for HLA-restricted tumor associated rejection antigens recognized by cytotoxic T lymphocytes. Some of these target antigens may be potentially useful for cancer specific immunotherapy. The expression of MAGE genes has been reported not only in melanoma but also in various other malignant tumors such as hepatocellular carcinoma and germ cell tumors.
Cellular localization
Cytoplasmic
Alternative names
- MAGE1 antibody
- Antigen MZ2 E antibody
- MAGE 1 antigen antibody
see all
Database links
- Entrez Gene: 4100 Human
- Omim: 300016 Human
- SwissProt: P43355 Human
Anti-MAGE 1 antibody images:
Western blot - MAGE 1 antibody (ab53131)
All lanes : Anti-MAGE 1 antibody (ab53131) at 1/300 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide
Predicted band size : 34 kDa
Observed band size : 34 kDa
Immunohistochemistry (Paraffin-embedded sections) - MAGE 1 antibody (ab53131)
ab53131, at 1/50 dilution, staining MAGE 1 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunising peptide.
References for Anti-MAGE 1 antibody (ab53131)
ab53131 has not yet been referenced specifically in any publications.
Publishing research using ab53131? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"