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Western blot - MAGEA10 antibody - Carboxyterminal end (ab71788)
Western blot - MAGEA10 antibody - Carboxyterminal end (ab71788)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGEA10 antibody - Carboxyterminal end (ab71788)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGEA10 antibody - Carboxyterminal end (ab71788)
(enlarge)


Product Name 

MAGEA10 antibody - Carboxyterminal end

 

See all MAGEA10 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MAGEA10 - Carboxyterminal end

Immunogen 

A KLH conjugated synthetic peptide selected from the C-terminal region of human MAGEA10.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, IHC-P, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). A range of other bands are detected from ~60-140 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

human breast carcinoma tissue and HL60 lysate.

Research areas 

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens

Relevance 

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.

MAGEA10 contains one MAGE domain. Its function is not known, though it may play a role in embryonal development and tumor transformation or aspects of tumor progression. MAGEA10 is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.

 

Alternative names MAGEA10 antibody - Carboxyterminal end (ab71788)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

4109    (Human)

Omim

    

300343    (Human)

SwissProt

    

P43363    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAGEA10 antibody - Carboxyterminal end is on this datasheet. But please do contact us if you would like any reassurance!


See below for MAGEA10 antibody - Carboxyterminal end images, references, products related to ab71788 and other tools.

 

MAGEA10 antibody - Carboxyterminal end images:

Product image  Western blot - MAGEA10 antibody - Carboxyterminal end (ab71788)

Western blot - MAGEA10 antibody - Carboxyterminal end (ab71788)

MAGEA10 antibody - Carboxyterminal end (ab71788) at 1/100 dilution + HL60 lysate at 12.5 µg

Predicted band size : 41 kDa
Observed band size : 41 kDa

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGEA10 antibody - Carboxyterminal end (ab71788)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAGEA10 antibody - Carboxyterminal end (ab71788)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue reacted with ab71788 at a 1:50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

 
 

Search PubMed (MEDLINE) for references to MAGEA10

 

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