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Products:Cancer >> Tumor immunology >> Tumor-associated antigens
Overview
Product name
Anti-MAGEA10 antibody
See all MAGEA10 products (2) ...
Description
Rabbit polyclonal to MAGEA10
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Cat, Dog
Immunogen
Synthetic peptide corresponding to a region within the internal sequence amino acids 320-369 (GSDPRSFPLW YEEALKDEEE RAQDRIATTD DTTAMASASS SATGSFSYPE) of human MAGEA10 (NP_001011543).
GSDPRSFPLW YEEALKDEEE RAQDRIATTD DTTAMASASS SATGSFSYPE
Positive control
Fetal heart lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified by peptide affinity chromatography method.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
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Western blot - MAGEA10 antibody (ab83557)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. MAGEA10 contains one MAGE domain. Its function is not known, though it may play a role in embryonal development and tumor transformation or aspects of tumor progression. MAGEA10 is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
Alternative names
- Cancer/testis antigen 1.10 antibody
- CT1.10 antibody
- MAGE 10 antigen antibody
see all
Database links
- Entrez Gene: 4109 Human
- Entrez Gene: 236852 Mouse
- Entrez Gene: 293832 Rat
- Omim: 300343 Human
- SwissProt: P43363 Human
Anti-MAGEA10 antibody images:
Western blot - MAGEA10 antibody (ab83557)
Anti-MAGEA10 antibody (ab83557) at 1 µg/ml + fetal heart lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 41 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
References for Anti-MAGEA10 antibody (ab83557)
ab83557 has not yet been referenced specifically in any publications.
Publishing research using ab83557? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"