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Western blot - MAGEA10 antibody (ab83557)
(enlarge)
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Product Name
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MAGEA10 antibody
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See all MAGEA10 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MAGEA10
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Immunogen
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Synthetic peptide corresponding to a region within the internal sequence amino acids 320-369 (GSDPRSFPLW YEEALKDEEE RAQDRIATTD DTTAMASASS SATGSFSYPE) of human MAGEA10 (NP_001011543).
BLAST 'GSDPRSFPLW YEEALKDEEE RAQDRIATTD DTTAMASASS SATGSFSYPE ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Fetal heart lysate.
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Research areas
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Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
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Relevance
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This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.
MAGEA10 contains one MAGE domain. Its function is not known, though it may play a role in embryonal development and tumor transformation or aspects of tumor progression. MAGEA10 is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Purification notes
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Purified by peptide affinity chromatography method.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAGEA10 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MAGEA10 antibody images, references, products related to ab83557 and other tools.
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MAGEA10 antibody images:
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 Western blot - MAGEA10 antibody (ab83557)
MAGEA10 antibody (ab83557) at 1 µg/ml + fetal heart lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 41 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
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Search PubMed (MEDLINE) for references to MAGEA10
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MAGEA10 antibody - more information
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Customer reviews (feedback) regarding MAGEA10 antibody |
Customer FAQs regarding MAGEA10 antibody |
Protocols for MAGEA10 antibody |
Price and availability of products related to MAGEA10 antibody |
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MAGEA10 antibody - related products:
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MAGEA10 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MAGEA10 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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